Anti-Kv2.1
- 貨號 Cat. No.
- APC-012
- 規格 Size
- 50 ml 0.2ml
Description
Anti-Kv2.1
| Product#: | APC-012 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
(CY)HMLPGGGAHGSTRDQSI, corresponding to residues 841-857 of rat Kv2.1 (Accession P15387). |
 |
| Putative epitope location: |
Intracellular, C-terminus. |
| Homology: |
Mouse - identical; human, rabbit - 15/17 residues identical. |
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
 | Western blotting of rat brain membranes: 1. Anti-Kv2.1 antibody (#APC-012) (1:500). 2. Anti-Kv2.1, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
| Kv2.1 Expression in Rat Pancreas |
 |
| Immunofluorescent staining of Kv2.1 in rat pancreas was visualized using Anti-Kv2.1 antibody (#APC-012) (1:50). Paraffin embedded sections of rat pancreas showing both endocrine (Islets of Langerhans) and exocrine areas. Strong and highly specific staining is evident only in endocrine cells (red). Secondary antibody is goat anti-rabbit Alexa 555 (1:400). Counterstain is Hoechst 33342 (blue). |
| References: |
| 1. Albrecht, B. et al. (1993) Receptors Channels 1:99. |
| 2. Murakoshi, H. and Trimmer, J.S. (1999) J Neurosci 19:1728. |
| 3. Archer, S.L et al. (1998) J Clin Invest 101, 2319. |
| 4. Escoubas, P. et al. (2002) Mol. Pharmacol. 62, 48. |
Kv2.1 is a member of the voltage-gated K+ channel superfamily. Together with the closely related Kv2.2 protein they form the Kv2 subfamily also known as Shab.1
As with all Kv channels, Kv2.1 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. The functional Kv channel is a tetramer that can either be a homotetramer or a heteromer of Kv2.1 and Kv2.2 subunits.
Both Kv2.1 and Kv2.2 channels are known as delayed rectifiers that is, channels that are activated by changes in membrane potential (depolarization) but inactivate very slowly. The current they form is known as IK or IDR. Accessory subunits such as KChaP and the electrically silent a subunits Kv8 and Kv9 can modulate biochemical and biophysical properties of Kv2.1.2
Kv2.1 is widely expressed throughout the body including brain, lung, pancreas, skeletal muscle and pulmonary artery.2
The main function of Kv2.1 is to maintain membrane potential and to modulate the electrical excitability in neurons and muscle. In rat pulmonary artery it probably mediates hypoxic pulmonary vasoconstriction together with the Kv9.3 subunit.3
Several toxins from spider venoms are potent blockers (affecting the channels in the nanomolar range) of KV2.1channels. Among these the most potent and selective are Stromatoxin-1 (ScTx-), (#RTS-350), (12.7nM)4 and Hanatoxin (42nM).see 4