Anti-Kv10.1 (EAG-1)
- 貨號 Cat. No.
- APC-104
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Kv10.1 (EAG-1)
| Product#: | APC-104 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
GDPAKRKGWARFKDAC, corresponding to residues 802-817 of rat KV10.1 (Accession Q63472). |
 |
| Putative epitope location: |
Intracellular, C-terminus. |
| Homology: |
Mouse ?identical, human-14 out of 16 residues identical. |
| Reactivity Confirmed: |
Rat, human. |
Applications:
| Western Blotting: |
 | Western blotting of rat brain lysate (1 and 3) and HEK-KV10.1 (2 and 4): 1 and 2. Anti- KV10.1 antibody (#APC-104) (1:200). 3 and 4. Anti- KV10.1 antibody, preincubated with the control antigen. |
| Immunocytochemistry: |
| Immunocytochemical staining of Kv10.1 in human MDA-468 mammary gland adenocarcinoma cells. |
 |
A. Paraformaldehyde fixed and permeabilized human MDA-468 mammary gland adenocarcinoma cells were stained with Anti- Kv10.1 antibody (#APC-104) (1:200) followed by goat-anti-rabbit-Alexa-555 secondary antibody (red staining). B. Nuclei were visualized with the cell permeable dye Hoechst 33342 (blue staining). C. Merge of (A) and (B). |
| References: |
| 1. Ludwig, J. et al. (1994) EMBO J. 13, 4451. |
| 2. Bauer, C. K. et al. (2001) J. Membrane Biol. 182, 1. |
| 3. Pardo, LA. et al. (1999) EMBO J. 18, 5540. |
KV10.1 is a member of the Ether-a-go-go family of voltage-activated K+ channels. To date, the family includes 8 members: the closely related KV10.2 (EAG-2) protein, the KV12 (elk) subfamily (elk1, elk2, elk3) and the KV11 (erg) proteins (erg1, erg2, erg3). As with the other KV superfamily members, KV10.1 shows the characteristic 6 transmembrane domains with intracelullar N and C termini.
The KV10 subfamily current is characterized by outward rectification without inactivation and slow activation kinetics. Its expression is normally confined to the brain where its physiological function has not yet been clarified.
Intriguingly, the KV10.1 channel has been implicated in malignant tumor development. It has been shown that KV10.1 was inappropriately expressed in several cancer cell lines. Moreover, one study showed that KV10.1 by itself had oncogenic potential, as a cell line transfected with the channel, induced aggressive and faster tumor growth in vivo as compared to a cell line transfected with an unrelated KV channel.
Alomone Labs offers an antibody raised against the cytoplasmic C terminal domain of the rat KV10.1 protein. The antibody has been tested against rat and human samples. The antibody is specific for KV10.1 and it will not crossreact with KV10.2.