- 貨號 Cat. No.
- APC-101
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Kv1.3 (extracellular)
(Voltage-gated potassium channel Kv1.3, Kcna3)
Epitope: |
Peptide KDYPASTSQDSFEA(C) corresponding to residues 263-276 of human Kv1.3. (accession number P22001). |
Putative epitope location: |
Extracellular, between S1 and S2 domains. |
Homology: |
Rat, mouse ?12 out of 14 residues identical. |
Reactivity Confirmed: |
Rat, Human. |
Applications:
Western Blotting: |
Rat brain membranes (1:200-1:500).
Indirect Flow Cytometry:
0.5-5mg antibody / 1x106cells.
|
 |
Western blotting of rat brain membranes:
1. Anti-Kv1.3 (extracellular) antibody (1:500)
2. Anti-Kv1.3 (extracellular) antibody, preincubated with the control peptide antigen. |
1. Chandy, K.G.et al. (2001) Toxicon 39, 1269.
|
2. Koo, G.C. et al. (1997) J Immunol. 158, 5120. |
Kv1.3 belongs to the Shaker family of voltage-dependent K+ channels.
The channel is widely expressed in the brain, lung and osteoclasts and in several cell
populations of hematopoietic origin. It is in these cells (particularly T lymphocytes) that
Kv1.3 function has centered a lot of attention. It was found that Kv1.3 is the main channel
responsible for maintaining the resting potential in quiescent cells and regulating the
Ca2+ signaling that is indispensable for normal T lymphocyte activation.
Based on the central role of Kv1.3 in regulating the initiation of an immune response,
the channel has been recognized as a potential target for immunosuppressant drugs.
The central role of Kv1.3 in immune system cells created a real need for a specific
antibody that would be able to work in flow cytometry applications.
Alomone has now developed an anti-Kv1.3 antibody directed against a specific
extracellular epitope of the channel.
The antibody can be obtained in a purified format (#APC-101) or labeled with
fluorescein (#APC-101-F). The antibodies have been tested in flow cytometry,
immunocytochemistry and western blot applications.
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