- 貨號 Cat. No.
- APC-101-F
- 規格 Size
- 50 μl
Description
Anti-Kv1.3 (extracellular)-FITC
(Voltage-gated potassium channel KV1.3, Kcna3)
Epitope: |
Peptide KDYPASTSQDSFEA(C) corresponding to residues 263-276 of human KV1.3. (accession number P22001). |
Putative epitope location: |
Extracellular, between the S1 and S2 domains. |
Homology: |
Rat, mouse ?12 out of 14 residues identical. |
Reactivity Confirmed: |
Rat, Human. |
Applications:
Flow Cytometry: |
FACS analysis of live intact Jurkat (human T cell leukemia) cells (0.5-5 mg antibody /1x106cells): |
 |
Unstained cells (pink line).
Cells + anti-KV1.3-extracellular -FITC (green line).
|
1. Chandy, K.G.et al. (2001) Toxicon 39, 1269.
|
2. Koo, G.C. et al. (1997) J Immunol. 158, 5120. |
3. Grissmer, S. et al. (1994) Mol. Pharmacol. 45, 1227. |
4. Garcia-Calvo, M. et al. (1993) J. Biol. Chem. 268, 18866. |
5. Garcia, M. L. et al. (1994) Biochemistry 33, 6834. |
6. Koschak, A. et al. (1998) J. Biol. Chem. 273, 2639. |
7. Kalman, K. et al. (1998) J. Biol. Chem. 273, 32697. |
KV1.3 belongs to the Shaker family of voltage-dependent K+ channels.
The channel is widely expressed in the brain, lung and osteoclasts and in several cell
populations of hematopoietic origin. It is in these cells (particularly T lymphocytes) that
KV1.3 function has centered a lot of attention. It was found that KV1.3 is the main channel
responsible for maintaining the resting potential in quiescent cells and regulating the
Ca2+ signaling that is indispensable for normal T lymphocyte activation.1,2
Based on the central role of KV1.3 in regulating the initiation of an immune response,
the channel has been recognized as a potential target for immunosuppressant drugs.1
KV1.3 channels are potently inhibited by several venomous peptide toxins among them
Charybdotoxin (#RTC-325, 2.6nM), Noxiustoxin (#RTN-340, 1nM), Kaliotoxin (#RTK-370, 0.65nM),
Margatoxin (#RTM-325, 0.05nM), Agitoxin-1 (#RTA-150, 1.7nM), Agitoxin-2 (#RTA-420, 0.004nM),
Hongotoxin-1 (#RTH-400, 0.09nM) and Stichodactyla toxin (#S-400, 0.01nM).3-7
The central role of KV1.3 in immune system cells created a real need for a specific
antibody that would be able to work in flow cytometry applications.
Alomone has now developed an anti-KV1.3 antibody directed against a specific
extracellular epitope of the channel.
The antibody can be obtained in a purified format (#APC-101) or labeled with
fluorescein (#APC-101F). The antibodies have been tested in flow cytometry,
immunocytochemistry and western blot applications.
詢價 / 洽詢此產品
