Anti-Kv1.1
- 貨號 Cat. No.
- APC-009
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Kv1.1
| Product#: | APC-009 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Epitope: |
GST fusion protein with sequence HRETEGEEQAQLLHV SSPNLASDSDLSRRSSSTISKSEYMEIEEDMNNSIAHYRQANIRT GNCTTADQNCVNKSKLLTDV, corresponding to residues 416-495 of mouse KV1.1 (Accession P16388). |
 |
| Putative epitope location: |
Intracellular, C-terminus. |
Applications:
| Western Blotting: |
 | Western blotting of rat brain membranes: 1.Anti- KV1.1 (1:200) 2.Anti- KV1.1, preincubated with the control antigen. |
| Immunocytochemistry: |
Rat brain sections |
| References: |
| 1. Temple, B. L., et al. (1988) EMBO J. 7, 2457. |
| 2. Gutman, G. A. et al. (2005) Pharmacol. Rev. 57, 473. |
| 3. Adelman, J. P. et al. (1995) Neuron 15, 1449. |
| 4. Bogin, O (2006) Modulator 21, 28. http://www.alomone.com/System/UpLoadFiles/DGallery/Docs/venom_toxins.pdf |
KV1.1 is a mammalian voltage dependent K+ channel, homologous to the Drosophilae Shaker K+ channel. KV1.1 was the first mammalian KV channel to be cloned from mouse brain.1 Eight Shaker related genes exist in mammals constituting the KV1, subfamily of the large KV channel family of genes.2
A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function.
The structure of KV1.1 channel is similar to all KV channels and includes six membrane spanning helixes creating a voltage sensor domain and a pore domain. 2
The channel is expressed in neurons and cardiac and skeletal muscle tissue as well as in retina and pancreas.2 The functional channel is considered low voltage activated and shows very little inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle. Mutations in the coding of KV1.1 gene were discovered in Episodic Ataxia patients.3
KV1.1 channels are sensitive to low doses of TEA (0.3 mM) and 4-AP (0.29 mM), the î™…lassical?non-selective potassium channel blockers.
Several venomous toxins from Snakes, Scorpions and Sea anemones are potent blockers (affecting the channels in the nanomolar range) of KV1.1 channels. Among these the most potent and selective are a-Dendrotoxin ((D-350, 0.4-4nM) and d-Dendrotoxin (D-380, 0.03-1.8nM), Dendrotoxin-K (D-400, 0.03nM), Agitoxin-2 (RTA-420, 0.044nM) and Hongotoxin-1 (RTH-400, 0.031nM).4