Anti-KLB Antibody

Anti-KLB Antibody LS-B3568

KLB Rabbit anti-Human Polyclonal (Internal) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B3568 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3568 was determined to be 10 ug/ml. ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. Learn more

KLB Antibody Details

Target: Human KLB

Host: Rabbit

Reactivity: Human, Mouse, Rat

Clonality: IgG Polyclonal 

Purification: Immunoaffinity Chromatography

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (10)

Western blot (WB)

Optimal dilution to be determined by the researcher.

 

KLB Antibody Specificity

Immunogen: KLB antibody was raised against synthetic peptide from internal region of human KLB (NP_783864.1, Q86Z14). Percent identity by BLAST analysis: Human (100%); Dog, Horse (92%); Rabbit, Chicken (85%); Guinea pig (84%).

Epitope: Internal

Specificity: Human KLB

KLB Antibody Packaging

Presentation: Lyophilized from PBS with 2% sucrose

Storage: Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

Restrictions: For research use only.