Anti-Kir6.2
- 貨號 Cat. No.
- APC-020
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Kir6.2
A Polyclonal Antibody to Kir6.2 (KATP) Inward Rectifier K+ Channel
| Product#: | APC-020 |
| Sizes: |
| 50 ml |
| 0.2 ml |
Kir6.2 is a member of the inward rectifier K+ channels (Kir channels), a large family of
voltage-independent K+ channels largely involved in stabilization of the membrane resting
potential and in K+ transport across membranes. Kir channels can be modulated by a variety of
intracellular agents such as protons, GTP-binding proteins and adenine nucleotides.
The ATP-sensitive channel (KATP) is especially important since it couples cellular
metabolism (intracellular ATP levels) with cell excitability. KATP channels have been
described in pancreatic b-cells, neurons, heart, skeletal and smooth muscle.
The KATP channel is composed of a Kir6.2 or Kir6.1 subunit and a sulphonylurea
receptor (SUR) subunit.
The pancreatic KATP channel for example, is composed of a complex of Kir6.2 and
SUR1 subunits, while the cardiac KATP channel is composed of Kir6.2 and SUR2A complexes.
Impaired b-cell KATP channel function due to mutations in either Kir6.2 or SUR1 subunits has been
linked to the recessive autosomal disorder called persistent hyperinsulinemic hypoglycemia
of infancy (PHHI). In addition, a Kir6.2 variant has recently been linked to an increased risk
of developing type-2 diabetes.
| Host: |
| Rabbit. |
| Epitope: |
| Peptide (C)SVAVAKAKPKFSIS, corresponding to amino acid residues 372-385 of rat Kir6.2 (accession number P70673). |
 |
| Putative epitope location: |
| Intracellular, C-terminal part. |
| Homology: |
| Mouse - identical; human- 12/14 residues identical. |
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
| . |
 | Western blotting of rat pancreas membranes: 1. Anti-Kir6.2 antibody (1:200). 2. Anti-Kir6.2 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
 |
| Staining of rat pancreas with Anti-Kir6.2 antibody (#APC-020). (A) Strong granular staining in a number of cells within the Islets of Langerhans is readily detected (red). (B) The negative control slide shows no staining. |
| References: |
| 1. Babenko, A.P. et al. (1998) Annu. Rev. Physiol. 60, 667. |
| 2. Huopio, H. et al. (2002) Am. J. Physiol. Endocrinol. Metab. 283, 207. |