Anti-Kir3.4 (GIRK4)
- 貨號 Cat. No.
- APC-027
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Kir3.4 (GIRK4)
| Product#: | APC-027 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit |
| Epitope: |
RNAMNQDMEIGVT(C), corresponding to residues 6-18 of rat Kir3.4 (Accession P48548). |
 |
| Putative epitope location: |
Intracellular, N-terminus. |
| Homology: |
Mouse, human, pig ?identical. |
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
| . |
 | Western blotting of rat heart membranes: 1. Anti-Kir3.4 antibody (1:200). 2. Anti-Kir3.4 antibody, preincubated with the control antigen. |
| References: |
| 1. Mark, M.D. et al. (2000) Eur. J. Biochem. 267, 5830 |
| 2. Yamada, M. et al. (1998) Pharmacol. Rev. 50, 723. |
| 3. Wickman, K. et al. (1998) Neuron 20, 103. |
| 4. Jin, W. and Lu, Z. (1998) Biochemistry 37, 13291. |
Kir3.4 is a member of the G-protein regulated inward-rectifier K+ (GIRK) channel subfamily which is part of an inward-rectifier K+ channel superfamily. The GIRK subfamily comprises four members in mammals (Kir3.1- Kir3.4) that present the common topology of the inward-rectifier superfamily: two transmembrane domains flanking a highly conserved pore region with the N and C-terminus located intracellularly.
Kir3.4 and the other Kir3 family subunits can be activated by neurotransmitters and other factors via the activation of G-protein coupled receptors. Binding of the corresponding ligand to the G-protein receptor induces the dissociation of G a-GTP from the Gbg dimer. The latter directly binds to Kir3 and activates the channel.
Kir3.4 expression is largely confined to the heart, where it co-assembles with Kir3.1 to form the prototypical muscarinic-gated K+ channel KACh. Indeed, knockout mice for Kir3.4 showed impaired heart rate following vagal nerve stimulation.
We are pleased to introduce an antibody directed against the intracellular N-terminus domain of the rat Kir3.4. Anti-Kir3.4 antibody (#APC-027) will also react with Kir3.4 of mouse, human and pig origin.
A peptide toxin originating from the Apis mellifera bee venom, Tertiapin (RTT-250) was shown to be a potent blocker of Kir3.4 containing channels (8.6nM for the Kir3.1/3.4 combination).4