Anti-KIF20A Antibody

Anti-KIF20A Antibody LS-B4028

KIF20A Rabbit anti-Human Polyclonal (Internal) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B4028 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4028 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500. Learn more

KIF20A Antibody Details

Target: Human KIF20A

Host: Rabbit

Reactivity: Human

Clonality: IgG Polyclonal 

Purification: Immunoaffinity Chromatography

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (5)

Western blot (WB) (1)

Optimal dilution to be determined by the researcher.

 

KIF20A Antibody Specificity

Immunogen: KIF20A antibody was raised against synthetic peptide from internal region of human KIF20A (NP_005724.1, O95235). Percent identity by BLAST analysis: Human, Dog, Rabbit, Horse, Pig (100%); Chicken (92%); Rat (86%).

Epitope: Internal

Specificity: Human KIF20A

KIF20A Antibody Packaging

Presentation: Lyophilized from PBS with 2% sucrose

Storage: Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

Restrictions: For research use only.