Anti-K2P9.1
- 貨號 Cat. No.
- APC-044
- 規格 Size
- 50 μl 0.2ml
Description
Anti-K2P9.1
(Acid-sensitive K+ channel 3, KCNK9, TASK3)
| Product#: | APC-044 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
| Peptide (C)DDYQQLELVILQSEPHR, corresponding to amino acid residues 57-73 of rat K2P9.1 (accession number Q923V6). |
| Putative epitope location: |
| Extracellular, near the P1 loop. |
 |
| Homology: |
| Human-15/17 residues identical. |
| Reactivity Confirmed: |
| Rat, human. |
Applications:
| Western Blotting: |
 | Proteins, lines 1 and 4 rat cerebellum lysate, lines 2 and 5 MCF-10A human mammary gland (fibrocystic disease) and lines 3 and 6 MCF-7 human mammary gland (adenocarcinoma), were separated in a SDS-PAGE gel and sequentially probed with Anti-K2P9.1 antibody (upper panel) and anti-b actin to ensure equal loading (lower panel). In lines 4,5 and 6 the antibody was preincubated with the control peptide antigen. Note that the expected 50 kD MW band is present in cerebellum and in the tumorigenic breast cancer cell line (MCF-7) but not in the non-tumorigenic breast cell line (MCF-10A). |
Control antigen included in price
| References: |
1. | Lesage, F. and Lazdunski, M. (2000) Am J Physiol. 279, F793. |
2. | Patel, A.J. et al. (2001) Trends Neurosci. 24, 339. |
3. | Mu, D. et al. (2003) Cancer Cell 3, 297. |
4. | Lauritzen, I. et al. (2003) J. Biol. Chem. 278, 232068. |
K2P9.1 (also named TASK3 or KCNK9) is a member of the 2-pore (2P) domain
K+ channels family that probably assemble as dimers to constitute a functional
channel.1 These channels show little time or voltage dependence and are
considered to be eak?or ackground?K+ channels, thereby generating
background currents which help set the membrane resting potential and cell excitation.
The channels are regulated by diverse physical and chemical stimuli including
temperature, pH, mechanical stretch, inhalation anesthetics, etc.2 The physiological
role of the K2P channels is not yet clear, though they were proposed to participate in
breathing, aldosterone secretion and anesthetic-mediated neuronal activity.
In human, expression of K2P9.1 is restricted in brain and its expression overlaps
with the structurally related K2P3.1 (TASK1, KCNK3) channel.
In rat, it is more widely expressed in other tissues as well as in brain.
Unexpectedly, K2P9.1 was found to be over-expressed between 5 to 100-fold
in 44% of breast cancer tumors tested.
Transfection and over-expression of K2P9.1 in cell lines promotes tumor growth and
confers resistance to hypoxia and serum deprivation.3 Recently, K2P9.1 was found
to play an important role in K+-dependent apoptosis of cerebellar granules.4
Alomone Labs is pleased to present a new polyclonal antibody to Anti-K2P9.1 (TASK3).
The antibody was raised against an epitope corresponding to residues 57-73 of the rat
K2P9.1 protein. The chosen epitope is located in the extracellular region near to the
Pore 1 loop, and shares 15 out of 17 residues with the human isoform.
Reactivity of the antibody was confirmed by Western blotting in both rat and human samples.