Anti-K2P5.1 (TASK-2)
- 貨號 Cat. No.
- APC-037
- 規格 Size
- 50 μl 0.2ml
Description
Anti-K2P5.1 (TASK-2)
| Product#: | APC-037 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
Peptide (C)YEQLMNEYNKANSPKGT, amino acid residues 483-499 of human K2P5.1 (Accession number O95279). |
 |
| Putative epitope location: |
Intracellular, C-terminus. |
| Homology: |
Mouse - 14/17 residues identical. |
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
 | Western blotting of rat kidney membranes: 1. Anti-K2P5.1 (1:200). 2. Anti-K2P5.1, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
 |
| Immunohistochemical staining of K2P5.1 in rat pancreas was visualized using Anti-K2P5.1 antibody (#APC-037) (1:50). Paraffin embedded sections of rat pancreas showing both endocrine (Islets of Langerhans) and exocrine areas. Strong and highly specific staining is evident only in endocrine cells (arrow). Universal Immuno-alkaline-phosphatase Polymer followed by New Fuchsin Substrate (Histofine, Nichirei Corp.) was used for the color reaction. Counterstain is Hematoxilin. |
| References: |
| 1. Reyes, R. et al. (1998) J. Biol. Chem. 273, 30863. |
| 2. Lesage, F. and Lazdunski, M. (2000) Am. J. Physiol. Renal Physiol. 279, F793. |
| 3. Warth, R. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 8215 |
K2P5.1 (also named TWIK-Related Acid Sensitive K+ channel 2, TASK-2 or KCNK5) is a member of the 2-pore (2P) domain K+ channels family that includes at least 16 members. These channels show little time or voltage dependence and are considered to be eak?or ackground?K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.
The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C termini. The functional channel is believed to be a dimer.
K2P channels are regulated by diverse physical and chemical stimuli including temperature, changes in intracellular pH, mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators.
As indicated by its name, K2P5.1 is sensitive to variations in external pH. K2P5.1 current is maximal at pH 8.8 but it is only 10% at pH 6.5.
The channel is expressed in several epithelial tissues including pancreas, lung, small intestine and specially kidney. In the kidney K2P5.1 has an important function in NaHCO3 absorption in the proximal tubule as knockout mice for K2P5.1 display metabolic acidosis and hypotension.