Anti-K2P2.1 (TREK-1)
- 貨號 Cat. No.
- APC-047
- 規格 Size
- 50 μl 0.2ml
Description
Anti-K2P2.1 (TREK-1)
| Product#: | APC-047 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
PeptideDPKSAAQNSKPRLSFSTK(C), corresponding to residues 8-25 of human K2P2.1 (TREK-1) (Accession Q9NRT2). |
 |
| Putative epitope location: |
Intracellular, N-terminus. |
| Homology: |
Rat-identical; mouse - 17/18 residues identical. |
| Reactivity Confirmed: |
| Rat, human. |
Applications:
| Western Blotting: |
 | Western blotting of rat brain membranes: 1. Anti-K2P2.1 (TREK-1) antibody (1:200). 2. Anti-K2P2.1, preincubated with the control peptide antigen. |
| References: |
| 1. Maingret, F. et al. (1999) J. Biol. Chem. 274, 26691. |
| 2. Lesage, F. and Lazdunski, M. (2000) Am. J. Physiol. Renal Physiol. 279, F793. |
| 3. Kim, D. (2003) Trends Pharmacol. Sci. 24, 648. |
| 4. Heurteaux, C. et al. (2006) Nat. Neurosci. 9, 1134. |
K2P2.1 (also named TWIK-Related K+ channel, TREK-1 or KCNK2) is a member of the 2-pore (2P) domain K+ channels family that includes at least 16 members. These channels show little time or voltage dependence and are considered to be eak?or ackground?K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.
The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C termini.
K2P channels are regulated by diverse physical and chemical stimuli including temperature, changes in intracellular pH, mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators. K2P2.1 can be integrated to a K2P subfamily that includes K2P4.1 (TRAAK) and K2P10.1 (TREK2) that are activated by intracellular unsaturated fatty acids such as arachidonic acid, lysophosphatidic acid and mechanical stretch. In addition, K2P2.1 can also be activated by general anesthetics such as halothane and chloroform and intracellular acidification.
K2P2.1 expression in humans is largely restricted to the brain with some expression in ovary and small intestine while K2P2.1expression in rodents is more widespread.
Recently, it has been shown that K2P2.1 has an important role in mood regulation, as knockout mice show resistance to depression, suggesting that K2P2.1 may be a potential target for anti-depressants.