Anti-K2P10.1

Anti-K_2P10.1

(TWIK-related K⁺ channel 2, KCNK10, TREK2)

Product#:

APC-055

Sizes:

50 ml

0.2 ml

K_2P10.1 is a member of the 2-pore (2P) domain K⁺ channels.  

These K⁺ channels also also known as background or eak?channels and help to set the resting membrane potential.  

K_2P channels can be activated by a wide variety of stimuli including temperature, pH, mechanical stretch, 

inhalation anesthetics, etc. The channels can also be subclassified based on their specific activators.  

K_2P10.1 can be integrated to a K_2P subfamily which includes K_2P2.1 (TREK1) and K_2P4.1 (TRAAK) that are  

activated by intracellular unsaturated fatty acids such as arachidonic acid, low intracellular pH and  

mechanical stretch. K_2P10.1 mRNA expression has been detected in the brain (primarily in the cerebellum)  

and in peripheral tissues such as pancreas and kidney. 



We have just released an Anti-K_2P10.1 antibody (#APC-055) directed against an epitope located  

at the C-terminal intracellular region of the rat protein. The chosen epitope is 19 out of 20 amino acids identical  

to the human K_2P10.1 protein.  

Host:

Rabbit.

Epitope:

Peptide TFRNYSLDEEKKEDETEKMC,   corresponding to amino acid residues 475-494 of rat K2P10.1 (accession number Q9JIS4).

Putative epitope location:

Intracellular, C-terminal domain.

Homology:

Human-19/20 residues identical.

Reactivity Confirmed:

Rat, human.

Applications:

Western Blotting:

Rat cerebellum lysate (1:200).

Western blotting of rat cerebellum lysate:  1. Anti-K2P10.1 antibody (1:200).    2. Anti-K2P10.1, preincubated with the control peptide antigen.

Immunohistochemistry:

Rat brain sections.

Immunohistochemical staining of TREK2 in rat cerebellum using Anti-K2P10.1 antibody (#APC-055)   (Green fluorescence, A). Purkinje cells were stained using mouse anti calbindin D28K   (Red, B). Double labeling revealed that TREK2-immunoreactive fibers (arrows) traverse the Purkinje layer and   run across the molecular layer (C).

References:

1. Kim, D. (2003) Trends Pharmacol. Sci. 24, 648.

2. Lesage, F. et al. (2000) J. Biol. Chem. 275, 28398.

3. Bang, H. et al. (2000) J. Biol. Chem. 275, 17412.