Anti-K2P1.1 (TWIK-1)
- 貨號 Cat. No.
- APC-110
- 規格 Size
- 50 μl 0.2ml
Description
Anti-K2P1.1 (TWIK-1)
| Product#: | APC-110 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
PeptideRQELRKLKRRFLEEHEC, corresponding to amino acid residues 53-69 of human K2P1.1 (Accession O00180). |
 |
| Putative epitope location: |
Extracellular, near the P1 loop. |
| Homology: |
| Rat, mouse, pig - identical. |
| Reactivity Confirmed: |
| Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blotting of rat brain lysate:
|
 | Western blotting of HEK-K2P1.1 transfected cells: 1. Anti-K2P1.1 antibody (1:300). 2. Anti-K2P1.1 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
| Mouse cerebellum sections. |
 |
Staining of mouse cerebellum with Anti-K2P1.1 antibody (#APC-110). A) K2P1.1 channel appears in glial processes (red) . B) staining of Purkinje nerve cells with mouse anti calbindin D28K (a calcium binding protein, green). C) Confocal merge of K2P1.1 channel and calbindin D28K demonstrates the separate localization of these proteins. |
| References: |
| 1. Lesage, F. et al. (1996) EMBO J. 15, 1004. |
| 2. Lesage, F. and Lazdunski, M. (2000) Am. J. Physiol. Renal Physiol. 279, F793. |
| 3. Goldstein, S.A.N. et al. (2003) Nat. Rev. Neurosci. 2, 175. |
K2P1.1 (also named TWIK-1 or KCNK1) is a member of the 2-pore (2P) domain K+ channels family that at the moment includes 14 members. These channels show little time or voltage dependence and are considered to be eak?or ackground?K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation. The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C termini.
K2P channels are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, etc. but are insensitive to the classical K+ channel blockers.
K2P1.1 was the first of the K2P channels to be identified and as its original name indicates (Tandem of P domains in a Weak Inward rectifier K+ channel) the channel behaves as a weak inward rectifier when expressed in heterologous systems. The channel is ubiquitously expressed with the most prominent expression in the brain, kidney and heart.
We have now produced a novel antibody directed against a highly conserved epitope in the extracellular region of the human K2P1.1 channel. The antibody will also react with rat and mouse samples.