Anti-IP3 Receptor-1
- 貨號 Cat. No.
- ACC-019
- 規格 Size
- 50 μl 0.2ml
Description
Anti-IP3 Receptor-1
| Product#: | ACC-019 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit |
| Epitope: |
Peptide(C)RIGL LGHPP HMNVN PQQPA corresponding to residues 2732-2750 of rat IP3R1 (Accession P29994). |
 |
| Putative epitope location: |
| C-terminus. |
| Homology: |
| Human, mouse - identical. |
| Reactivity Confirmed: |
| Rat and mouse. |
Applications:
| Western Blotting: |
 | Western blotting of rat brain membranes: 1. Anti-IP3R1 antibody (#ACC-019) (1:2000). 2. Anti-IP3R1 antibody, preincubated with the control. |
| Immunohistochemistry: |
| Expression of IP3R1 in mouse cerebellum |
 |
Immunohistochemical staining of IP3R1 in mouse cerebellum using Anti-IP3R1 antibody, (#ACC-019) in the mouse cerebellum (A). Immunoreactivity appeared in Purkinje cells (arrows in A) and their dendritic trees. In addition, axonal processes coarsing through cerebellar white matter were visualized (arrow in B). |
| References: |
| 1. Blondel, O. et al. (1993) J. Biol. Chem. 268, 11356. |
| 2. Va?rnai, P. et al. (2005) PNAS. 102, 7859. |
| 3. Cai, W. et al. (2004) J. Biol. Chem. 279, 23691. |
Inositol 1,4,5-triphosphate (IP3) serves as a second messenger for many neurotransmitters, hormones and growth factors.1 The binding of IP3 to its receptor (IP3R), which is a ligand gated Ca2+ channel, located predominantly at the endoplasmic reticulum, results in a rapid release of Ca2+ from intracellular stores.1
To date, three known isoforms of IP3R are known (designated IP3R1, IP3R2, and IP3R3) that can work as homotetramers or hetrotetramers. All three receptors have six transmembrane domains and a pore domain between TM5 and TM6. The N-terminus as well as the C-terminus are facing the cytoplasm. Each IP3R consists of an N-terminal ligand binding domain (LBD) and a C-terminal domain which is linked by a long regulatory domain. The C-terminus is constitutively active, suggesting that the regulatory domain is required in order to maintain the suppression of channel activity.2
IP3R1 is one of the most important channels responsible for Ca2+ release from intracellular stores and was shown to be the predominant isoform expressed in the central nervous system. IP3R1 is a pivotal player in many neuronal functions such as neuronal plasticity, neurite extension and others.3
Alomone Labs offers an antibody raised against an intracellular epitope of rat IP3R1.