Anti-Insulin Antibody

Anti-Insulin Antibody LS-B3300

Insulin Mouse anti-Human Monoclonal (D3E7) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3300 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3300 was determined to be 10 ug/ml. Learn more

Insulin Antibody Details

 

 

Target:

Human Insulin

 

Host:

Mouse

 

Reactivity:

Bovine, Human, Mouse, Porcine, Rat

 

Clonality:

IgG1,k Monoclonal [D3E7]

 

Purification:

Protein A Column

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml)

 

Immunohistochemistry - Frozen (IHC-Fr)

 

Immunofluorescence (IF)

 

ELISA

 

Optimal dilution to be determined by the researcher.

 

Insulin Antibody Specificity

 

Specificity:

Kd for this antibody is 6.3 x 10-8M. This antibody is specific for both insulin and proinsulin Species cross-reactivity: Cross-reacts with Human, bovine, Mouse, porcine and Rat.

Insulin Antibody Packaging

 

Presentation:

PBS, pH 7.4, 0.1% sodium azide

 

Storage:

Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

 

Restrictions:

For research use only.