Anti-Infliximab ELISA Kit
- 規格 Size
- 96 T
- 品牌 Brand
- AntibodySystem
Description
PRINCIPLE OF THE ASSAY
This assay employs the quantitative sandwich enzyme immunoassay technique. Infliximab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Infliximab will be captured by immobilized Infliximab. After washing away any unbound substances, a biotin-labeled Infliximab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Infliximab bound in the initial step. The color development is stopped and the intensity of the color is measured.
This assay employs the quantitative sandwich enzyme immunoassay technique. Infliximab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Infliximab will be captured by immobilized Infliximab. After washing away any unbound substances, a biotin-labeled Infliximab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Infliximab bound in the initial step. The color development is stopped and the intensity of the color is measured.
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