Anti-ICAM-1 Antibody

Anti-ICAM-1 Antibody LS-B3431

ICAM-1 Mouse anti-Human Monoclonal (15.2) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3431 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3431 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Learn more

ICAM-1 Antibody Details

 

 

Target:

Human ICAM-1

 

Host:

Mouse

 

Reactivity:

Human

 

Clonality:

IgG1 Monoclonal [15.2]

 

Purification:

Protein G Column

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml)

 

Immunohistochemistry - Frozen (IHC-Fr)

 

Immunoprecipitation (IP)

 

Flow Cytometry (Flo) (1:50 - 1:100)

 

Optimal dilution to be determined by the researcher.

 

ICAM-1 Antibody Specificity

 

Immunogen:

ICAM1 antibody was raised against human monocytes.

 

Specificity:

Recognizes the human CD54 cell surface antigen, a 90kD glycoprotein also known as ICAM-1. CD54 is expressed by many cells following activation by inflammatory mediators. Clone 15.2 is reported to block CD54 function.

ICAM-1 Antibody Packaging

 

Presentation:

PBS, pH 7.4, 0.09% sodium azide.

 

Storage:

+4°C or -20°C, Avoid repeated freezing and thawing.

 

Restrictions:

For research use only.