Anti-Human p75NTR
- 貨號 Cat. No.
- ANT-007
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Human p75NTR
| Product#: | ANT-007 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Epitope: |
PeptideCEEIPGRWITRSTPPE corresponding to residues 188-203 of human p75NTR (SwissProt accession P08138). |
 |
| Putative epitope location: |
Extracellular, stalk domain. |
| Homology: |
Mouse-identical, rat 15 out of 16 residues identical. |
| Reactivity Confirmed: |
Rat, human. |
Applications:
| Western Blotting: |
Rat brain and A875 human melanoma cells (1:200). |
 | Western blotting of rat brain membranes: 1. Anti-human p75 NTR, 1:200. 2. Anti-human p75 NTR, preincubated with the control peptide antigen. |
 | Western blotting of human melanoma cells A875: 1. Anti-human p75 NTR, 1:200. 2. Anti-human p75 NTR, preincubated with the control peptide. |
 | Expression of p75NTR in normal rat tissue (right blot) and in human cancer cell lines (left blot). p75NTR was visualized with Anti-Human p75NTR antibody (#ANT-007) diluted 1:200. Note that human cancer cell lines from hemapoietic origin show high p75NTR expression, while cell lines from prostate and colon cancer origin show lower levels. Interestingly, p75NTR from rat (right blot and the C6 cell line) and human (left blot) samples run with a different apparent MW, probably due to species-specific differential glycosylation. |
| Immunohistochemistry: |
| Staining of nerve growth factor receptor (NGFR) with Anti-human p75NTR (#ANT-007) in rat brain. In (A), cells in the diagonal band are stained positive for NGFR. In (B), staining of the same section shown in (A), with goat Anti-ChAT confirms that NGFR staining was specific to choinergic neurons (see below). |
 |
Staining of nerve growth factor receptor (NGFR) with Anti-human p75NTR (#ANT-007) in rat brain. In (A), cells in the nucleus basalis mangocellularis are stained positive for NGFR. In (B), staining of the same section shown in (A), with goat Anti-ChAT confirms that the NGFR staining was specific to cholinergic neurons (see below). |
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FACS analysis of A875 human melanoma cells: |
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| Immunocytochemistry: |
Staining of human melanoma cells A875 with Anti-p75NTR (#ANT-007). Cells were incubated with Anti-p75NTR (I:200) (A and B) or with Anti-p75NTR preincubated with the control antigen (C and D), followed by goat-anti-rabbit-Alexa Fluor 555 (Red). (x100). |
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| Immunoprecipitation: |
 | Immunoprecipitation of 3T3/p75 transfected cells with Anti-human p75NTR- extracellular:
Red arrows indicate the p75NTR receptor while the black arrow shows the IgG heavy chain. Immunoblot was performed with the Anti-human p75NTR-extra antibody. |
| References: |
| 1. Hempstead, B.L. (2002) Curr. Opin. Neurobiol. 12, 260. |
| 2. Chao, M.V. (2003) Nat. Rev. Neurosci. 4, 299. |
| 3. Barker, P.A. (2004) Neuron 42, 529. |
The p75NTR receptor is a member of the TNF receptor superfamily. p75NTR, like all the superfamily members is a type I transmembrane protein with tandem cysteine-rich domains in the extracellular portion and a intracellular death domain. p75NTR binds its ligands as a homodimer but can also form heterodimers with other receptors such as TrkA, TrkB, TrkC, Nogo receptor and sortilin. The precise multimeric receptor complex formed between p75NTR and the other receptors will determine the ligand being recognized (see below) and the biological response to its binding.
As it name implies, p75NTR binds all the neurotrophins (NGF, BDNF, NT-3 and NT-4) with similar nM affinities. Co-expression of p75NTR with the Trk receptors enhances the ability to bind and respond to the specific neurotrophin and induces cell survival. On the other hand, it has recently been demonstrated that p75NTR binds with high affinity to the unprocessed form of NGF (proNGF) probably as a complex with sortilin and this leads to cell death by apoptosis. Finally, a multimeric complex of p75NTR and Nogo receptor binds myelin proteins such as Nogo-66, MAG and OmgP resulting in inhibition of axonal growth.
Alomone Labs has now developed a new antibody specific for the p75NTR (#ANT-007). The antibody was raised against a highly conserved epitope located in the stalk domain of the human p75NTR extracellular region. The chosen epitope makes this antibody highly suited for several applications including indirect flow cytometry, immunocytochemistry, immunohistochemistry and western blot. The antibody works equally well in mouse and rat samples.