Anti-human p75NTR - intracellular
- 貨號 Cat. No.
- ANT-011
- 規格 Size
- 50 μl 0.2ml
Description
Anti-human p75NTR - intracellular
| Product#: | ANT-011 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit |
| Epitope: |
PeptideCKQNKQGANSRPVNQT corresponding to residues 278-293 of human p75NTR (SwissProt accession P08138). |
 |
| Putative epitope location: |
Intracellular, juxtamembrane domain. |
| Homology: |
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
 | Western blotting of rat brain membranes: 1. Anti-human p75 NTR î ¢ntracellular (#ANT-011), 1:200. 2. Anti-human p75 NTR î ¢ntracellular, preincubated with the control peptide antigen. |
 | Western blotting of human melanoma cells A875: 1. Anti-human p75 NTR î ¢ntracellular (#ANT-011), 1:200. 2. Anti-human p75 NTR î ¢ntracellular, preincubated with the control peptide antigen. |
| References: |
| 1. Hempstead, B.L. (2002) Curr. Opin. Neurobiol. 12, 260. |
| 2. Chao, M.V. (2003) Nat. Rev. Neurosci. 4, 299. |
| 3. Barker, P.A. (2004) Neuron 42, 529. |
The p75NTR receptor is a member of the TNF receptor superfamily. p75NTR, like all the superfamily members is a type I transmembrane protein with tandem cysteine-rich domains in the extracellular portion and a intracellular death domain.
p75NTR binds its ligands as a homodimer but can also form heterodimers with other receptors such as TrkA, TrkB, TrkC, Nogo receptor and sortilin. The precise multimeric receptor complex formed between p75NTR and the other receptors will determine the ligand being recognized (see below) and the biological response to its binding.
As its name implies, p75NTR binds all the neurotrophins (NGF, BDNF, NT-3 and NT-4) with similar nM affinities. Co-expression of p75NTR with the Trk receptors enhances the ability to bind and respond to the specific neurotrophin and induces cell survival.
On the other hand, it has recently been demonstrated that p75NTR binds with high affinity to the unprocessed form of NGF (proNGF) probably as a complex with sortilin and this leads to cell death by apoptosis. Finally, a multimeric complex of p75NTR and Nogo receptor binds myelin proteins such as Nogo-66, MAG and OmgP resulting in inhibition of axonal growth.
We have now developed a new antibody specific for the p75NTR, Anti-human p75NTR-intracellular (#ANT-011). This antibody was raised against a highly conserved epitope located in the juxtamembrane domain of the human p75NTR intracellular region. The antibody works equally well in mouse and rat samples.