Anti-Human p75NTR (extracellular)-ATTO-550
- 貨號 Cat. No.
- ANT-007-AO
- 規格 Size
- 50 μl
Description
Anti-Human p75NTR (extracellular)-ATTO-550
| Product#: | ANT-007-AO |
| Sizes: |
| 50 µl |
| Host: |
| Rabbit. |
| Epitope: |
PeptideCEEIPGRWITRSTPPE corresponding to residues 188-203 of human p75NTR (Accession number P08138). |
 |
| Putative epitope location: |
| Extracellular, stalk domain. |
| Homology: | ||
Mouse-identical, rat 15 out of 16 residues identical.
|
Applications:
| Immunocytochemistry: |
 |
Immunocytochemical staining of p75NTR in p75NTR-transfected 3T3 cells. (A) Staining of intact live p75NTR-transfected 3T3 cells with Anti-human p75NTR (extracellular)-ATTO- 550 antibody (#ANT-007-AO) (1:100). (B) Live view of the same field as in (A). |
 |
Surface expression of p75NTR in rat dorsal root ganglion (DRG) cells. Intact live DRG neurons were labeled with Anti-mGluR1 (extracellular) antibody (#AGC-006) (1:100) followed by goat-anti-rabbit Alexa 488 secondary antibody (green). The cells were then labeled with Anti-human p75NTR (extracellular) ATTO-550 antibody (#ANT-007-AO) (1:50) (red). Nuclei were visualized using the cell-permeable DNA binding dye Hoechst 33342 (blue). Note that the Anti-human p75NTR (extracellular) ATTO-550 antibody does not stain all DRG neurons as expected. Colocalization is observed in some nerve fibers of the mGluR1 and p75NTR receptors. |
| References: |
| 1. Hempstead, B.L. (2002) Curr. Opin. Neurobiol. 12, 260. |
| 2. Chao, M.V. (2003) Nat. Rev. Neurosci. 4, 299. |
| 3. Barker, P.A. (2004) Neuron 42, 529. |
| 4. Dechant, G. and Barde, Y.A. (2002) Nat. Neurosci. 5, 1131. |
The p75NTR receptor is a member of the tumor necrosis factor (TNF) receptor superfamily. Like all members of this superfamily, p75NTR is a type I transmembrane protein with tandem cysteine-rich domains in its extracellular portion, as well as an intracellular death domain. p75NTR binds to its ligands as a homodimer but can also form heterodimers with other receptors such as TrkA, TrkB, TrkC, Nogo receptor, and sortilin. The precise multimeric receptor complex formed between p75NTR and the other receptors determine the ligand being recognized (see below) and the biological response to its binding.1-3
As its name implies, p75NTR binds to all the neurotrophins (NTR) (NGF, BDNF, NT-3, and NT-4) with similar nanomolar affinities. Co-expression of p75NTR with the Trk receptors enhances its ability to bind and respond to the specific neurotrophin and induces cell survival. On the other hand, it has recently been demonstrated that p75NTR binds with high affinity to the unprocessed form of NGF (proNGF), probably as a complex with sortilin, leading to cell death by apoptosis.1-3 Finally, a multimeric complex of p75NTR and Nogo receptor binds to myelin proteins such as Nogo-66, MAG, and OmgP, resulting in inhibition of axonal growth.4
Alomone Labs has developed a new antibody specific for the p75NTR. The antibody was raised against a highly conserved epitope located in the stalk domain in the extracellular region of the human p75NTR. The Anti-Human p75NTRantibody (#ANT-007) is highly suited for several applications including indirect flow cytometry, immunocytochemistry, immunohistochemistry, and western blotting.
We are pleased to offer a new version of this antibody that is directly labeled with an ATTO-550 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine.
The antibody has been tested in immunocytochemical and immunohistochemical applications and is specially suited for experiments requiring simultaneous labeling of different markers.