Anti-Human Orai1 (extracellular)-FITC
- 貨號 Cat. No.
- ACC-060-F
- 規格 Size
- 50 μl
Description
Anti-Human Orai1 (extracellular)-FITC
| Product#: | ACC-060-F |
| Sizes: |
| 50 µl |
| Host: |
Rabbit. |
| Epitope: |
| Peptide (C)KKQP GQPRP TSK, corresponding to amino acid residues 203-214 of human Orai1 (Accession Q96D31). |
| Putative epitope location: |
| 2nd extracellular loop. |
| Homology: |
| Canis ?identical; bovine ?12/13 amino acid residues identical. |
| Reactivity Confirmed: |
| Human. |
Applications:
| Western Blotting: |
| Jurkat (human T lymphocyte, acute T cell leukemia) and HL-60 (human promyelocytic leukemia) cell lines. |
 | Western blot analysis of Jurkat (lanes 1 and 2) and HL-60 (lanes 3 and 4) cell lysates: 1, 3. Anti-Human Orai1(extracellular) antibody (#ACC-060), (1:200). 2, 4. Anti-Human Orai1 (extracellular) antibody preincubated with the control peptide antigen. |
| Flow Cytometry: |
 |
Flow cytometry analysis of live Jurkat (human acute T cell leukemia) cells: ____ Unstained Jurkat cells. ____Jurkat cells stained with Anti-Human Orai1(extracellular)-FITC antibody (#ACC-060-F), (10 ç—¢ per 1x106 cells). |
| References: |
| 1. Eisner, D.A. et al. (2005) Exp. Physiol. 90, 3. |
| 2. Chakrabarti, R. and Chakrabarti, R. (2006) J. Cell. Biochem. 99, 1503. |
| 3. Feske, S. et al. (2006) Nature 441, 179. |
| 4. Pickett, J. (2006) Nat. Rev. Mol. Cell Biol.7,794. |
| 5. Luik, R.M. et al. (2006) J. Cell. Biol. 174, 815. |
| 6. Wu, M.M. et al. (2006) J. Cell. Biol. 174, 803. |
| 7. Ong, H.L. et al. (2007) J. Biol. Chem. 282, 9105. |
| 8. Luik, R.M. and Lewis, R.S. (2007) Trends Mol. Med. 13, 103. |
| 9. DeHaven, W.I. et al. (2007) J. Biol. Chem. 282,17548. |
| 10. Lis, A. et al. (2007) Curr. Biol. 17, 794. |
Cytosolic calcium (Ca+2) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth, and proliferation.1,2 The mechanism controlling intracellular Ca+2 level influx either by calcium-release-activated Ca+2 channels (CRAC) or from intracellular stores has become of great interest.
Recently, several key players of the store-operated complex have been identified.3 Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 acts as an endoplasmic reticulum (ER) Ca+2 sensor.3,4 The formation of functional channels requires the presence of both Orai1 and STIM1 proteins working as a complex that involves co-clustering of Orai1 on the plasma membrane with STIM1 on the ER.4-6 TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex.7 In T cells, Ca+2 entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore-forming subunit.3,8
Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N- and C-termini. In addition, two mammalian homologs of Orai1 have been identified: Orai2 and Orai3.3,9 All three (Orai1, Orai2, and Orai3) have been shown to be capable of forming store-operated channels.9,10
Alomone Labs is pleased to offer a new version of the Anti-Human Orai1 (extracellular) antibody (#ACC-060) directly conjugated to FITC. The Anti-Human Orai1 (extracellular)-FITC antibody (#ACC-060-F) can be used for immunofluorescent applications such as direct flow cytometry and recognizes Orai1 from human samples.