Anti-human IgM Antibody

Catalog Number

250187

Unit

0.1 mg

 

Format

Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A affinity chromatography.

Data and Images

Description

This antibody recognizes human m chains.The specificity of the anti-IgM antibody has been verified in indirect ELISA against human IgM, IgG, IgA and IgD. The antibody can be used for detecting human m heavy chains in ELISA and flow cytometry.

Product Citations

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Alternate Names

Immunoglobulin M, type μ heavy chain

Accession No.

Clonality

IIE2

Antigen

Application Notes

FACS with whole blood: 100 μl blood + 10 μl antibody

FACS with cells: 500,000 cells in 50 μl + 10 μl antibody

This antibody recognizes human μ heavy chains. In flow cytometry, the antibody stains >95% of Ramos cell (λ, μ, δ positive), >95% of Daudi cells (κ, μ, δ positive) and <10% of human peripheral blood mononuclear cells. The antibody does not react with IgG, IgA or IgD.

E: 1:500-1:1,000

Reacts with  :H

Storage

Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.