Anti-Human H4 Histamine Receptor (extracellular)

 

Anti-Human H₄ Histamine Receptor (extracellular)

Product#:

AHR-004

Sizes:

50 µl

0.2 ml

Host:

Rabbit.   Type: Polyclonal.

Epitope:

Peptide HTLFEWDFGKEIC, corresponding to amino acids 75-87 of human H4 Histamine Receptor (Accession Q9H3N8).

Putative epitope location:

1st extracellular loop.

Homology:

Wonî–¹ recognize rat or mouse H4 Histamine Receptor.

Reactivity Confirmed:

Human.

Applications:

Western Blotting:

Western blot analysis of human chronic myelogenous leukemia (K562) (lanes 1 and 3) and human promyelocytic leukemia (HL-60) (lanes 2 and 4) cell lysates:  1, 2. Anti-Human H4 Histamine Receptor (extracellular) antibody (#AHR-004) (1:400).  3, 4. Anti-Human H4 Histamine Receptor (extracellular), preincubated with the control peptide antigen

Flow Cytometry:

.

FACS analysis of H4 Histamine Receptor expression in live intact Jurkat (acute T-cell leukemia) cell lines. ___ Control cells + Goat-anti-Rabbit-PE.   ___ Cells+ Anti-Human H4 Histamine Receptor (extracellular) antibody (#AHR-004), (1:20) + Goat-anti-Rabbit-PE.

Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from

L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.¹

 

The biological effects of histamine are mediated through four receptors (H₁- H₄ Histamine receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.

H₄ Histamine Receptor couples to G_i/G₀ proteins and receptor activation leads to inhibition of adenylate cyclase, mobilisation of calcium from intracellular stores and activation of the mitogen-activated protein kinase (MAPK) cascade. ^{1, 2}

 

H₄ Histamine Receptor is largely expressed in haemopoietic cells including mast cells, eosinophils, dendritic cells and T lymphocytes. H₄ Histamine Receptors modulate eosinophil migration and the selective recruitment of mast cells leading to amplification of histamine-mediated immune responses. In addition, H₄ Histamine Receptors are involved in dendritic cell activation and the regulation of T lymphocyte cytokine production.^{2, 3}

These studies indicate that H₄ Histamine Receptor is an attractive therapeutic target for the treatment of inflammatory disorders, such as allergy, asthma, chronic pruritus and autoimmune diseases.^{2, 3}

 

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the extracellular 1^st loop of the human H₄ Histamine receptor. Anti-Human H₄ Histamine Receptor (extracellular) antibody (#AHR-004) can be used in Western blot and indirect flow cytometry applications, and recognizes H₄ Histamine receptor from human samples.