Anti-HSPD1 Antibody

Anti-HSPD1 Antibody LS-B3678

HSPD1 Mouse anti-Human Monoclonal () (LK-2) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3678 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3678 was determined to be 10 ug/ml. Learn more

HSPD1 Antibody Details

 

 

Target:

Human HSPD1

 

Host:

Mouse

 

Reactivity:

Bacteria, Bovine, Human, Mouse, Rat

 

Clonality:

IgG1 Monoclonal [LK-2]

 

Purification:

Protein G Column

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10)

 

Western blot (WB) (0.25)

 

Immunoprecipitation (IP) (10)

 

Optimal dilution to be determined by the researcher.

 

HSPD1 Antibody Specificity

 

Immunogen:

HSPD1 antibody was raised against human HSP60 produced through recombinant DNA methods in E. coli.

 

Specificity:

Detects an ~60 kD protein, corresponding to the apparent molecular mass of Hsp60 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, chicken, dog, guinea pig, hamster, monkey, pig, rabbit, plant (spinach), Borrelia, E. coli, helicobacter pylori, M. bovis (Hsp65), Salmonella typhimurium, Streptococcus pyogenes, Treponema hyodysenteriae, Treponema innocense, Trichinella spiralis, Yersinia entercolitica, and yeast origins. Deletion mutant analysis indicates that the epitope recognized by LK-2 is located between residues 383-419 of the human Hsp60 sequence (which corresponds to residues 356-393 of the mycobacterial Hsp60 sequence).

HSPD1 Antibody Packaging

 

Presentation:

PBS, pH 7.2, 0.1 mM PMSF, 50% glycerol

 

Storage:

Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

 

Restrictions:

For research use only.