Anti-HSP27 Antibody

Anti-HSP27 Antibody LS-B3412

HSP27 Mouse anti-Human Monoclonal (5F12-B10) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3412 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3412 was determined to be 10 ug/ml. Learn more

HSP27 Antibody Details

 

 

Target:

Human HSP27

 

Host:

Mouse

 

Reactivity:

Human, Monkey

 

Clonality:

IgG1 Monoclonal [5F12-B10]

 

Purification:

Protein G Column

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml)

 

Western blot (WB) (1:1000)

 

Optimal dilution to be determined by the researcher.

 

HSP27 Antibody Specificity

 

Immunogen:

Native human Hsp27 protein.

HSP27 Antibody Packaging

 

Presentation:

PBS, pH 7.2, 0.09% azide, 50%glycerol

 

Storage:

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

 

Restrictions:

For research use only.