Anti-HMGCS1 Antibody

Anti-HMGCS1 Antibody LS-B4016

HMGCS1 Rabbit anti-Human Polyclonal (Internal) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B4016 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4016 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500. Learn more

HMGCS1 Antibody Details

Target: Human HMGCS1

Host: Rabbit

Reactivity: Human, Mouse, Rat

Clonality: IgG Polyclonal 

Purification: Immunoaffinity Chromatography

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (5)

Western blot (WB) (1)

Optimal dilution to be determined by the researcher.

 

HMGCS1 Antibody Specificity

Immunogen: HMGCS1 antibody was raised against synthetic peptide from internal region of human HMGCS1 (NP_002121.4, Q01581). Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Rabbit, Horse, Pig (100%); Guinea pig (92%).

Epitope: Internal

Specificity: Human HMGCS1

HMGCS1 Antibody Packaging

Presentation: Lyophilized from PBS with 2% sucrose

Storage: Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

Restrictions: For research use only.