Anti-HLA-E Antibody

Anti-HLA-E Antibody LS-B3729

HLA-E Mouse anti-Human Monoclonal (MEM-E/06) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3729 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3729 was determined to be 10 ug/ml. Learn more

HLA-E Antibody Details

 

 

Target:

Human HLA-E

 

Host:

Mouse

 

Reactivity:

Human, Primate

 

Clonality:

IgG1 Monoclonal [MEM-E/06]

 

Purification:

Protein A Column

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10)

 

Immunoprecipitation (IP)

 

Flow Cytometry (Flo)

 

Optimal dilution to be determined by the researcher.

 

HLA-E Antibody Specificity

 

Immunogen:

HLA-E antibody was raised against bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.

 

Specificity:

Recognized native surface-expressed HLA-E, but not denatured heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results showed that the antibody cross-reacts with some classical MHC Class I molecules (HLA-A3, -A11, -B7). However, the recent Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that the antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32, -B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7

HLA-E Antibody Packaging

 

Presentation:

Tris buffered saline (TBS), 15 mM sodium azide, approx., pH 8.0.

 

Storage:

Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

 

Restrictions:

For research use only.