Anti-HLA-E Antibody

Anti-HLA-E Antibody LS-B3730

HLA-E Mouse anti-Human Monoclonal (MEM-E/07) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3730 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3730 was determined to be 10 ug/ml. Learn more

HLA-E Antibody Details

 

 

Target:

Human HLA-E

 

Host:

Mouse

 

Reactivity:

Human

 

Clonality:

IgG1 Monoclonal [MEM-E/07]

 

Purification:

Protein A Column

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10)

 

Immunoprecipitation (IP)

 

Flow Cytometry (Flo)

 

Optimal dilution to be determined by the researcher.

 

HLA-E Antibody Specificity

 

Immunogen:

HLA-E antibody was raised against bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.

 

Specificity:

Recognized native surface-expressed HLA-E, but not denatured heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly)

HLA-E Antibody Packaging

 

Presentation:

PBS, 15 mM sodium azide, approx., pH 7.4.

 

Storage:

Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

 

Restrictions:

For research use only.