Anti-HK2 / Hexokinase 2 Antibody

Anti-HK2 / Hexokinase 2 Antibody LS-B3571

HK2 / Hexokinase 2 Rabbit anti-Human Polyclonal (N-Terminus) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B3571 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3571 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. Learn more

HK2 / Hexokinase 2 Antibody Details

Target: Human HK2 / Hexokinase 2

Host: Rabbit

Reactivity: Human, Mouse, Rat

Clonality: IgG Polyclonal 

Purification: Immunoaffinity Chromatography

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (5)

Western blot (WB)

Optimal dilution to be determined by the researcher.

 

HK2 / Hexokinase 2 Antibody Specificity

Immunogen: HK2 / Hexokinase 2 antibody was raised against synthetic peptide from N-terminus of human HK2 (NP_000180.2, P52789). Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig (100%); Chicken, Zebrafish (85%).

Epitope: N-Terminus

Specificity: Human HK2 / Hexokinase 2

HK2 / Hexokinase 2 Antibody Packaging

Presentation: Lyophilized from PBS with 2% sucrose

Storage: Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

Restrictions: For research use only.