Anti-Histone H1 Antibody

Anti-Histone H1 Antibody LS-B4038

Histone H1 Mouse anti-Human Monoclonal (AE-4) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B4038 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4038 was determined to be 5 ug/ml. Learn more

Histone H1 Antibody Details

Target: Human Histone H1

Host: Mouse

Reactivity: Human

Clonality: IgG2a Monoclonal [AE-4] 

Purification: Protein G Column

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (5)

Immunohistochemistry - Frozen (IHC-Fr)

Western blot (WB) (1:100)

Immunofluorescence (IF)

ELISA

Optimal dilution to be determined by the researcher.

 

Histone H1 Antibody Specificity

Immunogen: Histone H1 antibody was raised against nuclei from myeloid leukemia biopsy cells.

Specificity: Recognizes histone H1. The histone proteins serve as a structural scaffold for the organization of nuclear DNA into chromatin. In eukaryotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 that assemble to form an octamer round which the DNA is wrapped in a sequence dependant manner. The resulting complex is known as a nucleosome and is the basic subunit of chromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity, links these nucleosomes together resulting in the 30 nm chromatin fiber. In blots of rat brain homogenate a duplet is observed at 27-29 kD consistent with Histone H1. Speckled staining is observed in the nucleus of normal and malignant cells.

Histone H1 Antibody Packaging

Presentation: PBS, 0.09% sodium azide.

Storage: +4°C or -20°C, Avoid repeated freezing and thawing.

Restrictions: For research use only.