Anti-Hdm2
- 貨號 Cat. No.
- A-153
- 規格 Size
- 100 μg
- 品牌 Brand
- Boston Biochem
Description
Anti-Hdm2
rabbit polyclonal
Product Information
100 μg
2 mg/ml in PBS pH 7.5, 0.1mg/ml BSA
Peptide region to the C-terminus of Hdm2.
Use & Storage
The use of BSA for blocking and incubation steps is recommended. Store unopened vial at -80oC. Avoid repeated freeze/thaw cycles.
Recommended dilution range for Western blot analysis is 2-5 μg/ml.
Catalog #: A-153
Anti-Hdm2
Product Source:
rabbit polyclonal
Mouse monoclonal antibody with high specificity for Hmd2 (Human double minute 2), a ubiquitin RING finger E3 ligase and major regulator of the tumor suppressor p53. The E3 ligase activity is confined to the C-terminal domain of Hdm2 and is responsible for the ubiquitination and subsequent proteasomal degradation of p53. Although the isolated RING domain is capable of p53 ubiquitination, other regions of the protein including a central acidic domain are also crucial for full E3 ligase activity. Hdm2 also regulates its own intracellular levels by auto-ubiquitination, and can be SUMOylated which decreases auto-ubiquitination activity but increases activity toward p53. This antibody recognized and epitope in the C-terminus of the protein and is useful for the detection of Hdm2 (E3-200) auto-ubiquitination.
Product Information
Quantity:
100 μg
Stock:
2 mg/ml in PBS pH 7.5, 0.1mg/ml BSA
Antigen:
Peptide region to the C-terminus of Hdm2.
Use & Storage
Storage:
The use of BSA for blocking and incubation steps is recommended. Store unopened vial at -80oC. Avoid repeated freeze/thaw cycles.
Specificity:
Recommended dilution range for Western blot analysis is 2-5 μg/ml.