Anti-HCN4
- 貨號 Cat. No.
- APC-052
- 規格 Size
- 50 μl 0.2ml
Description
Anti-HCN4
| Product#: | APC-052 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
GST fusion protein with sequence HGHLH DSAEE RRLIA EGDAS PGEDR TPPGL AAEPE RP, corresponding to amino acid residues 119-155 of human HCN4 (Accession Q9Y3Q4) |
 |
| Putative epitope location: |
Intracellular, N-terminus. |
| Homology: |
Rabbit - identical; rat - 35/37 amino acid residues identical; mouse - 33/37 amino acid residues identical. |
| Reactivity Confirmed: |
| Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of HCN4 in rat brain membranes: 1. Anti-HCN4 antibody (#APC-052) (1:200). 2. Anti-HCN4 antibody, preincubated with the control fusion protein. |
| Immunohistochemistry: |
Expression of HCN4 in Mouse Thalamus |
 |
Immunohistochemical staining of HCN4 channel in mouse thalamus using Anti-HCN4 antibody (#APC-052). A, HCN4 channel (red) appears in the neuropil of the lateral nucleus (LN). B, staining of reticular nucleus (RN) with mouse anti parvalbumin (a calcium binding protein, green). C, Confocal merge of HCN4 and parvalbumin images demonstrates separate localization of these proteins in the thalamus. |
| References: |
| 1. Much, B. et al (2003) J.Biol.Chem. 278, 43781. |
| 2. Notomi, T. et al (2004) J. Comp. Neurol. 471, 241. |
| 3. Gravante, B. et al (2004) J.Biol.Chem. 279, 43847. |
| 4. Moosmang, S. et al. (2001) Eur. J. Biochem. 268, 1646. |
| 5. Garcia-Frigola, C. et al. (2003) Gene Expr Patterns3,777. |
| 6. Monteggia, L. et al. (2000)Molecular. Brain. Research. 1-2, 129. |
Hyperpolarization-activated cation currents (Ih) appear in the heart and the brain and have a crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2
The Ih currents are generated by the Hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which is comprised of four homologous members, HCN1-4.
Each HCN subunit consists of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain for cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2
The HCN subunits can form functional homomers and can also co-assemble into functional heteromers.2
The channels are closely related to each other and share a homology of about 60%. However, their similarity decreases in the cytoplasmic N- and C-termini. The HCN1-4 channels mainly differ from each other in their speed of activation and the extent to which they are modulated by cAMP. HCN1, weakly affected by cAMP, is the fastest channel, followed by HCN2, HCN3 and HCN4.
HCN4 is highly expressed in a restricted manner in adult sinoatrial (SA) node, constituting a good
molecular marker for the adult cardiac pacemaker and might serve as a unique marker of the developing SA node .4,5 mRNA expression of HCN4 is most abundant in medial habenula and anterior and principal relay nuclei of the thalamus.6
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the HCN4 channel. The Anti-HCN4 (#APC-052) can be used for Western Blotting and Immunohistochemistry applications. It has been designed to recognize HCN4 from human, rat and mouse samples.