Anti-HCN2
- 貨號 Cat. No.
- APC-030
- 規格 Size
- 50 μl 0.2ml
Description
Anti-HCN2
| Product#: | APC-030 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit
|
| Epitope: |
Peptide (C)EEAG PAGEP RGSQ AS, corresponding to amino acid residues 147-161 of human HCN2 (Accession Q9UL51). |
 |
| Putative epitope location: |
| Intracellular, N-terminus. |
| Homology: |
| Rat, mouse - identical. |
| Reactivity Confirmed: |
Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of HCN2 in rat brain membranes: 1. Anti-HCN2 antibody (#APC-030), (1:200). 2. Anti-HCN2 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of HCN2 in rat cerebellum |
 |
Immunochistochemical staining of HCN2 channel in rat cerebellum frozen section using Anti-HCN2 antibody (#APC-030 ). A, HCN2 channel (red) appears in Purkinje cells (arrows). B, staining of astrocytes with mouse anti glial fibrillary acidic protein (GFAP, green) demonstrates the restriction of HCN2 to neuronal cell bodies. C, Confocal merge of HCN2 and GFAP images demonstrates the respective localization of these proteins. Expression of HCN2 in Mouse hypothalamus |
 |
Immunohistochemical staining of HCN2 channel in mouse hypothalamus using Anti-HCN2 antibody (#APC-030). A, HCN2 channel (red) appears in cells of the paraventricular nucleus (PVN, arrows). B, Staining of paraventricular nerve cells with mouse anti calcium binding protein, CBD28k (green). C, Confocal merge of HCN2 and CBD28k demonstrates some co-localization of these proteins in the PVN. (V = Third ventricle). |
| Immunocytochemistry: |
Expression of HCN2 in rat Dorsal Root Ganglion (DRG) |
 |
Immunocytochemical staining of HCN2 in rat DRG primary culture using Anti-HCN2 antibody (#APC-030).(green). Paraformaldehyde-fixed and permeabilized rat DRG cells were stained with Anti-HCN2 antibody (1:100) followed by Alexa-488-conjugated goat-anti-rabbit secondary antibody (green). Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342 (blue). |
| References: |
| 1. Much, B. et al. (2003) J.Biol.Chem. 278, 43781. |
| 2. Notomi, T. et al. (2004) J. Comp. Neurol. 471, 241. |
| 3. Hofmann, F. et al. (2005) Pharmacol. Rev.57,455. |
Hyperpolarization-activated cation currents (Ih) appear in the heart and the brain having crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2
The Ih currents are generated by the Hyperpolarization-activated cyclic nucleotide-gated cannel family (HCN), which comprises of four homologous members, named HCN1-4. Each HCN subunit consist of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain to cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2
The HCN subunits can form functionally homomers and can also co-assemble into functionally heteromers.2
The channels are closely related to each other and share homology of about 60%. However, they are diverging from each other in their cytoplasmic N- and the C-terminus. The channels HCN1-4 mainly differ from each other with regard to their speed of activation and the extent by which they are modulated by cAMP. HCN1 is the fastest channel, followed by HCN2, HCN3 and HCN4.2,3
HCN2 is the most abundant neuronal channel and is found almost ubiquitously in the brain.3
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the HCN2 channel. The Anti-HCN2 (#APC-030) can be used for Western Blotting, Immunohistochemical and immunocytochemical applications. It has been designed to recognize HCN2 from human, rat and mouse samples.