Anti-HCN1
- 貨號 Cat. No.
- APC-056
- 規格 Size
- 50 μl 0.2ml
Description
Anti-HCN1
| Product#: | APC-056 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
Peptide (C)KPNSASNSRDDGNSVYPSK, corresponding to amino acid residues 6-24 of rat HCN1(Accession Q9JKB0). |
 |
| Putative epitope location: |
| Intracellular, N-terminous. |
| Homology: |
Mouse - 18/19 amino acid residues identical; human - 16/19 amino acid residues identical. |
| Reactivity Confirmed: |
| Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of HCN1 in rat brain membrane proteins: 1. Anti-HCN1 antibody (#APC-056), (1:200). 2. Anti-HCN1 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
| Expression of HCN1 in Mouse Cerebellum |
 |
Immunohistochemical staining of HCN1 in the mouse cerebellum using Anti-HCN1 antibody (#APC-056). A, HCN1 (green) appears in the cerebellar pinceau. B, Calcium binding calbindinD28-K (red), a marker of Purkinje neurons, is stained in the same section. C, Merge of the images demonstrates the position of the HCN1-positive pinceau structures at the axon initial segment of Purkinje neurons. |
| Immunocytochemistry: |
Expression of HCN1 in rat Dorsal Root Ganglion (DRG) |
 |
Immunocytochemical staining of HCN1 in rat DRG primary culture using Anti-HCN1 antibody (#APC-056). Paraformaldehyde-fixed and permeabilized rat DRG cells were stained with Anti-HCN1 antibody (1:300) followed by Alexa-488-conjugated goat-anti-rabbit secondary antibody (green). Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342 (blue). |
| References: |
| 1. Much, B. et al (2003) J.Biol.Chem. 278, 43781. |
| 2. Notomi, T. et al (2004) J. Comp. Neurol. 471, 241. |
| 3. Gravante, B. et al (2004) J.Biol.Chem. 279, 43847 |
Hyperpolarization-activated cation currents (Ih) appear in the heart and the brain and have a crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2
The Ih currents are generated by the Hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which is comprised of four homologous members, HCN1-4.
Each HCN subunit consists of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain for cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2
The HCN subunits can form functional homomers and can also co-assemble into functionally heteromers.2
The channels are closely related to each other and share a homology of about 60%. However, their similarity decreases in the cytoplasmic N- and C-termini. The channels HCN1-4 mainly differ from each other in their speed of activation and the extent to which they are modulated by cAMP. HCN1, weakly affected by cAMP, is the fastest channel, followed by HCN2, HCN3 and HCN4.
HCN1 is extensively expressed in the brain, in specific areas like the neocortex, hippocampus, cerebellum and superior colliculus.2,3
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the HCN1 channel. The Anti-HCN1 (#APC-056) can be used for Western Blotting, Immunohistochemical and immunocytochemical applications. It has been designed to recognize HCN1 from human, rat and mouse samples.