Anti-H3 Histamine Receptor
- 貨號 Cat. No.
- AHR-003
- 規格 Size
- 50 μl 0.2ml
Description
Anti-H3 Histamine Receptor
| Product#: | AHR-003 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
| Peptide (C)RTRLRLDGGREAGPE, corresponding to amino acids 228-242 of rat H3 Histamine Receptor (Accession Q9QYN8). |
 |
| Putative epitope location: |
| 3rd intracellular loop. |
| Homology: |
Mouse- identical; human 14 out of 16 residues identical. |
| Reactivity Confirmed: |
Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blotting of rat (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): 1, 2. Anti- H3 Histamine Receptor antibody (#AHR-003), (1:200). 3, 4. Anti-H3 Histamine Receptor, preincubated with the control peptide antigen.
|
| Immunohistochemistry: |
Expression of H3 Histamine Receptor in rat cerebellum |
 |
Immunohistochemical staining of Rat brain frozen sections with Anti-H3 Histamine Receptor antibody (#AHR-003), (1:100), (green). A. H3 Histamine Receptor is particularly expressed in dendrites of Purkinje cells (arrows). B. Staining with mouse anti-parvalbumin (red) detected Purkinje cells and interneurons in the molecular layer. C. Merge of the two images demonstrates that the staining was restricted to dendrites of Purkinje cells.Cell nuclei were labeled with DAPI (blue) as the counterstain. |
Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1
The biological effects of histamine are mediated through four receptors (H1- H4 Histamine receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.
H3 Histamine Receptor couples to Gi/G0 proteins and receptor activation leads to inhibition of adenylate cyclase, activation of the mitogen-activated protein kinase (MAPK) cascade and inhibition of the Na+/H+ exchanger. 1, 2
H3 receptors are expressed primarily in the central nervous system (CNS) where they are located in presynaptic membranes of histaminergic neurons, where they negatively regulate the synthesis and release of histamine. In addition, H3 receptors are also located on nonhistaminergic neurons, where they regulate the release of other amines such as dopamine, serotonin, and norepinephrine. 2,3
Based on these studies, a central role for H3 receptors has been proposed in disorders involving cognition such as attention deficit and hyperactivity disorder (ADHD), Alzheimer disease and schizophrenia as well as sleep and energy homeostasis (i.e. obesity) disorders.2, 3
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the intracellular 3rd loop of the rat H3 Histamine receptor. Anti-H3 Histamine Receptor antibody (#AHR-003) can be used in Western blot and immunohistochemical applications, and recognizes H3 Histamine receptor from rat, mouse and human samples.