Anti-H1 Histamine Receptor

 

Anti-H₁ Histamine Receptor

Product#:

AHR-001

Sizes:

50 µl

0.2 ml

Host:

Rabbit. Type: Polyclonal.

Epitope:

Peptide (C)HSRQYVSGLHLNRE, corresponding to amino acids 396-409 of rat H1 Histamine Receptor (Accession P31390).

Putative epitope location:

Intracellular, 3rd loop.

Homology:

Mouse- identical; human, dog- 13/ 14 residues identical.

Reactivity Confirmed:

Rat, mouse.

Applications:

Western Blotting:

Western blotting of rat heart membranes (lanes 1 and 3) and rat basophilic leukemia (RBL) cell lysates (lanes 2 and 4):        1, 2. Anti- H1 Histamine Receptor antibody (#AHR-001) (1:200).        3, 4. Anti- H1 Histamine Receptor, preincubated with the control peptide. antigen

Western blotting of mouse brain membranes: 1. Anti- H1 Histamine Receptor antibody (#AHR-001) (1:400). 2. Anti- H1 Histamine Receptor, preincubated with the control peptide antigen.

Immunohistochemistry:

Expression of H1 Histamine receptor in mouse brain. Staining of H1 histamine receptor in mouse ventromedial hypothalamus (VMH) with Anti-H1 histamine receptor antibody (#AHR-001). A. H1 histamine receptor (green fluorescence) appears in the outline of the VMH nucleus (arrows). B. DAPI (blue) counterstain labels all cell nuclei including VMH.

Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from

L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.¹

 

The biological effects of histamine are mediated through four receptors (H₁- H₄ Histamine receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.

H₁ receptors couple to G_q/G₁₁ proteins leading to phospholipase C activation, inositol phosphate production and calcium mobilization.¹

 

H₁ receptors are expressed in many cell types including endothelial and smooth muscle cells where they mediate vasodilatation and increased vascular permeability effects.

H₁ receptors are widely distributed through the brain where the mediate sleep-wake cycles and regulate cognitive functions.

H₁ receptors are expressed in a several cell populations involved in the immune response, including T-cells and B-cells, monocytes and eosinophiles. ^2,3

 

H₁ anti-histamine compounds have been available for almost thirty years and are among the most commonly used medication for conditions such as allergic inflammation. In addition, H₁ anti-histamines are also prescribed for the treatment of insomnia and analgesia.^{2, 3}

 

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the intracellular 3^rd loop of the rat H₁ Histamine receptor. Anti-H₁ Histamine receptor antibody (#AHR-001) can be used in Western blot and immunohistochemical applications, and recognizes H₁ Histamine receptor from rat, mouse and human samples.