Anti-GDNF
- 貨號 Cat. No.
- ANT-014
- 規格 Size
- 50 μl 0.2ml
Description
Anti-GDNF
| Product#: | ANT-014 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
| Peptide (C)DDNLVYHILRKHSAKR, corresponding to amino acid residues 192-207 of human GDNF (precursor) (Accession P39905). |
 |
| Putative epitope location: |
| C-terminus of the mature GDNF. |
| Homology: |
Mouse, dog, monkey, bovine ?identical. Rat ?15/16 amino acid residues identical. |
| Reactivity Confirmed: |
| Human, rat. |
Applications:
| Western Blotting: |
Western blot analysis of recombinant human GDNF (hGDNF). Lanes 1 and 4: 50 ng hGDNF. Lanes 2 and 5: 10 ng hGDNF. Lane 3: 4 ng hGDNF. Lanes 1-3: Anti-GDNF antibody (#ANT-014), (1:1000). Lanes 4-5: Anti-GDNF antibody preincubated with the control peptide antigen. |
 |
| Immunohistochemistry: |
 |
Expression of GDNF in rat dorsal root ganglion (DRG). A longitudinal frozen section of rat DRG was incubated overnight at 4èš“ with Anti-GDNF antibody (#ANT-014), (1:50) followed by Alexa 555-conjugated goat anti-rabbit antibody (red staining). Hoechst 33342 (blue) was used as a counterstain. Staining is specific for neurons. Note that Schwann cells as well as satellite glial cells are both negative. |
 |
Staining of glia-derived neurotrophic factor (GDNF) in rat hippocampus. A. Immunohistochemical staining of rat hippocamous frozen sections with Anti-GDNF antibody (#ANT-014)(1:100)(red) is apparent in the pyramidal layer (asterik), in astrocytic fibers (horizontal white arrows) and in some interneurons (down-pointing arrow) B. Calbindin D28k (green) appears in a subset of cells in the pyramidal layer. C. Merge of GDNF and calbindin demonstrates some co-localization in neurons. The blue counterstain is DAPI. |
| Immunocytochemistry: |
 |
Immunocytochemical staining of GDNF in rat C6 glioma cells. A. Paraformaldehyde-fixed and permeabilized rat C6 glioma cells were stained with Anti-GDNF antibody (#ANT-014), (1:200) followed by Alexa 555-conjugated goat anti-rabbit antibody (red staining). Nuclei were visualized with the cell-permeable dye Hoechst 33342 (blue staining). B. Live view of the same field as in (A). |
| References: |
| 1. Lin, L.F.et al.(1993) Science 260, 1130. |
| 2. Airaksinen, M.S. and Saarma, M. (2002) Nat. Rev. Neurosci. 3, 383. |
| 3. Sariola, H. and Saarma, M. (2003) J. Cell Sci. 116, 3855. |
| 4. Paratcha, G. et al. (2003) Cell 113, 867. |
Glial cell-line derived neurotrophic factor (GDNF) is a neurotrophic factor originally identified for its ability to promote the survival of dopaminergic neurons in the midbrain.1
GDNF is part of the neurotrophic factor family known as GDNF-family ligand (GFL), which includes neurturin (NRTN), artemin (ARTN), and persephin (PSPN).2,3
The GFL ligands are structurally related to the transforming growth factor-b (TGF-b) protein superfamily and contain seven cysteine residues with the same relative spacing as other members of this family.2
Typical of neurotrophic factors, GDNF is produced in the form of a precursor, preproGDNF, which is then cleaved during secretion to become proGDNF, and later processed to the mature (homodimeric) GDNF form.2
The GFLs convey their activity by binding to a multicomponent receptor that includes the RET receptor tyrosine kinase and a glycosyl phosphatidylinositol (GPI)-linked ligand-bindingsubunit known as GDNFfamily receptor a (GFRa).2,3
Four different GFRa subunits have been identified (GFRa 1-4) that determine the specificity of the GFRa-RET receptor complex. Thus, GFRa1 together with RET make up the high affinity receptor for GDNF.
The GDNF and GFRa complex interaction with RET induces activation of the intracellular tyrosine kinase domain of the latter. Phosphorylated RET can then interact with several intracellular signaling cascades including MAP kinase, PI3K and PLCg signaling pathways.2,3
Lately a RET-independent signaling pathway for GDNF has been shown to involve binding of GDNF and GFRa to the neural cell adhesion molecule (NCAM). Signaling of GDNF via the NCAM complex stimulates intracellular tyrosine kinases such as Fyn and FAK.4
GDNF is synthesized and secreted by a wide variety of cell types in the central and peripheral nervous system where it exerts its neurotrophic effects in the development and maintenance of distinct sets of neurons. Accordingly, GDNF knockout mice die shortly after birth, reflecting the central role of GDNF in neurodevelopment.2
Remarkably, GDNF was found to be an essential morphogen for kidney formation as well as to have a role is spermatogenesis.3
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located near the C-terminal end of human GDNF. Anti-GDNF antibody (#ANT-014) can be used in Western blot, immunocytochemical, and immunohistochemical applications and recognizes GDNF from human and rat samples.