Anti-Galanin Receptor Type 2
- 貨號 Cat. No.
- AGR-012
- 規格 Size
- 50 µl 0.2 ml
Description
Anti-Galanin Receptor Type 2
| Product#: | AGR-012 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
Peptide(C)GSGSQRAKRKVTR, corresponding to amino acid residues 223-235 of mouse Galanin receptor type 2 (Accession O88854). |
 |
| Putative epitope location: |
| 3rd intracellular loop. |
| Homology: |
Rat ?identical; human ?11/13 amino acid residues identical. |
| Reactivity Confirmed: |
| Rat, mouse, human. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat hippocampus (lanes 1 and 4), DRG (lanes 2 and 5), brain (lanes 3 and 6), mouse brain (lanes 7 and 9) and human prostate carcinoma LNCaP cell lysates: 1, 2, 3, 7, 8. Anti-Galanin Receptor Type 2 antibody (#AGR-012), (1:200). 4, 5, 6, 9, 10. Anti-Galanin Receptor Type 2 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of GALR2 in rat DRG |
 |
Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-Galanin Receptor Type 2 antibody (#AGR-012), (1:100).GALR2 is expressed in the DRG neurons. Hoechst 33342 is used as the counterstain. |
Galanin is a neuropeptide, consisting of 29-30 amino acid residues, involved in the regulation of cognition, mood, feeding, seizure activity and pain. Galanin exerts its action via three known Galanin Receptors, the Galanin Receptor Type 1 (GALR1), Galanin Receptor Type 2 (GALR2) and Galanin Receptor Type 3 (GALR3). The Galanin Receptors belong to the G-Protein Coupled Receptor superfamily.1-4
GALR2 is widely expressed in the brain (with highest levels of mRNA found in the hypothalamus, hippocampus, amygdala and pyriform cortex) and in peripheral tissues such as the small intestine, heart, kidney, and liver. GALR2 high levels of expression were also found in rat dorsal root ganglia.1
GALR2 activation increases inositol phosphate hydrolysis, mediates the release of Ca2+ into the cytoplasm from intracellular stores, and opens Ca2+-dependent chloride channels in a PTX-resistant manner, indicating that GALR2 couples to Gq/11 proteins.5
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of mouse Galanin Receptor type 2 (GALR2). The Anti-Galanin Receptor Type 2 antibody (#AGR-012) can be used for Western blot analysis and immunohistochemistry applications. It has been designed to recognize GALR2 from mouse, rat and human samples.