Anti-Galanin Receptor Type 1
- 貨號 Cat. No.
- AGR-011
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Galanin Receptor Type 1
| Product#: | AGR-011 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
Peptide RSKPGKPRSTTN(C) corresponding to amino acid residues 60-71 of mouse GALR1 (Accession P56479). |
 |
| Putative epitope location: |
| 1st intracellular loop. |
| Homology: |
Rat and human ?Identical. |
| Reactivity Confirmed: |
Rat. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat brain lysate: 1. Anti-GALR1 antibody (#AGR-011), (1:200). 2. Anti-GALR1 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
| Expression of GALR1 in Rat Dorsal Root Ganglia (DRG) |
 |
| Immunohistochemical staining of frozen section of rat DRG using Anti-GALR1 antibody (#AGR-011), (1:200) (red). Galanin Recpetor is expressed in DRG neuron cells. Counterstain is Hoechst 33342 (blue). |
| Immunocytochemistry: |
Expression of GALR1 in Rat Dorsal Root Ganglion primary culture |
 |
Immunocytochemical staining of GALR1 in a primary culture of rat dorsal root ganglion (DRG) neurons. A, D. A paraformaldehyde-fixed and permeabilized DRG primary culture was stained with Anti-GALR1 antibody (#AGR-011), (1:100) followed by Alexa-555-conjugated goat anti-rabbit secondary antibody (red). B, E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342 (blue). C. Merged images of panels A and B. F. Merged images of panels D and E. |
Galanin is a neuropeptide, consisting of 29-30 amino acid residues, involved in the regulation of cognition, mood, feeding, seizure activity and pain. Galanin exerts its action via three known Galanin receptors, the Galanin receptor type 1 (GALR1), Galanin receptor type 2 (GALR2) and Galanin receptor type 3 (GALR3). The Galanin receptors belong to the G-protein-coupled receptor superfamily.1-4
Based on mRNA, the GALR1 is predominantly distributed in the central nervous system, spinal cord and dorsal root ganglia while GALR2 is abundant in both central and peripheral nervous system. GALR3 mRNA is distributed in peripheral tissues such as heart, spleen, and testis. However, its expression in the brain is still controversial.4,5
Alomone Labs is pleased to offer a highly specific antibody directed against mouse Galanin Receptor type 1 (GALR1). The Anti-GALR1 antibody (#AGR-011) can be used for Western blot analysis, Immunohistochemical and Immunocytochemical applications. It has been designed to recognize GALR1 from mouse, rat and human samples.