Anti-GABRB3 Antibody

Anti-GABRB3 Antibody LS-B3610

GABRB3 Goat anti-Human Polyclonal (Internal) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B3610 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D3, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3610 was determined to be 2.5 ug/ml. Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Preliminary experiments in Human Brain (Cerebral Cortex, Frontal Cortex and Cerebellum) lysates gave no specific signal but low background (at antibody concentration up to 1 ug/ml). Learn more

GABRB3 Antibody Details

Target: Human GABRB3

Host: Goat

Reactivity: Bovine, Dog, Human, Mouse, Rat

Clonality: Polyclonal 

Purification: Immunoaffinity Chromatography

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (2.5)

Western blot (WB)

ELISA (1:8000)

Optimal dilution to be determined by the researcher.

 

GABRB3 Antibody Specificity

Immunogen: GABRB3 antibody was raised against synthetic peptide C-EKTAKAKNDRSK from an internal region of human GABRB3 (NP_000805.1; NP_068712.1). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (100%), Orangutan (100%), Gibbon (100%), Monkey (100%), Marmoset (100%), Mouse (100%), Rat (100%), Hamster (100%), Elephant (100%), Panda (100%), Cat (100%), Dog (100%), Horse (100%), Bovine (92%), Rabbit (92%), Pig (92%), Lizard (92%), Opossum (83%), Turkey (83%), Platypus (83%).

Epitope: Internal

Specificity: Human GABRB3. This antibody is expected to recognize both reported isoforms (NP_000805.1, NP_068712.1).

GABRB3 Antibody Packaging

Presentation: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

Storage: Store at -20°C. Minimize freezing and thawing.

Restrictions: For research use only.