Anti-GABA(A)a6 Receptor (extracellular)
- 貨號 Cat. No.
- AGA-004
- 規格 Size
- 50 μl 0.2ml
Description
Anti-GABA(A)a6 Receptor (extracellular)
| Product#: | AGA-004 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
| Peptide QLEDEGNFYSENVSRILDN(C) corresponding to amino acid residues 20-37 of rat GABA(A)a6 receptor (Accession P30191). |
 |
| Putative epitope location: |
| N-terminus, Extracellullar. |
| Homology: |
| Mouse - identical; human - 17/19 amino acid residues identical. |
| Reactivity Confirmed: |
| Rat, mouse. |
Applications:
| Western Blotting: |
| Rat brain membranes (1:200). |
 | Western blot analysis of GABA(A)a6 receptor in rat brain membrane: 1. receptor (extracellular) antibody (#AGA-004), (1:200). 2. Anti- GABA(A)a6 receptor (extracellular) antibody, preincubated with the control peptide antigen. Anti-GABA(A)a6 |
| Immunohistochemistry: |
Expression of GABA(A)a6 receptor in mouse cerebellum |
 |
Immunohistochemical staining of GABA(A)a6 receptorin mouse cerebellum using Anti- GABA(A)a6 receptor (extracellular) antibody (#AGA-004). A, GABA(A)a6 receptor (red) appears in the granule layer (white asterik). B, Parvalbumin (green) appears in Purkinje cells and in the molecular layer. C, merge of GABA(A)a6 receptor and parvalbumin indicates that GABA(A)a6 receptor is restricted to granule cells. The blue counterstain is DAPI. |
| References: |
| 1. Owens,D.F. and Kriegstein, A.R. (2002) Nature Rev. Neurosci. 3, 715. |
| 2. Whiting, P.J. (1999) Neurochem. Inter. 34, 387. |
| 3. Loh, E.W and Ball, D. (2000) Neurochem. Int. 37, 413. |
| 4. Surendran, S. et al. (2003) Mol. Gen. Met. 80, 74. |
GABA (g-aminobutyric acid) is the major inhibitory neurotransmitter in the brain.
Its production, release, reuptake and metabolism occur in the nervous system.1
The GABA transmitter interacts with two major types of receptors: ionotropic GABA(A) and the metabotropic GABA(B) receptors. The GABA(A) receptor belongs to the ligand gated ion channel superfamily.2
The GABA(A)R, is a heteropentamer, with all of its five subunits contributing to the pore formation.
To date, eight subunit isoforms were cloned: a, b, g, d, e, P, q, and r.1 Six a subunit isoforms
were described to exist in mammals (a1-a6). The native GABA(A) receptor, in most cases,
consists of 2a, 2b and 1g subunit. The a subunit is the most common subunit and is expressed ubiquitously.
It determines the affinities for allosteric ligands shown by GABA(A)R.
A possible role for the GABA(A) receptors in alcohol action has been derived from animal studies, in vitro cell models and human research which suggested an important role for GABA(A)a6 along with b2, a1 and g2, in alcohol related phenotypes.3
In Canavan disease (inherited leukodystrophy), low level of expression of GABA(A)a6 was reported.4
Its production, release, reuptake and metabolism occur in the nervous system.1
The GABA transmitter interacts with two major types of receptors: ionotropic GABA(A) and the metabotropic GABA(B) receptors. The GABA(A) receptor belongs to the ligand gated ion channel superfamily.2
The GABA(A)R, is a heteropentamer, with all of its five subunits contributing to the pore formation.
To date, eight subunit isoforms were cloned: a, b, g, d, e, P, q, and r.1 Six a subunit isoforms
were described to exist in mammals (a1-a6). The native GABA(A) receptor, in most cases,
consists of 2a, 2b and 1g subunit. The a subunit is the most common subunit and is expressed ubiquitously.
It determines the affinities for allosteric ligands shown by GABA(A)R.
A possible role for the GABA(A) receptors in alcohol action has been derived from animal studies, in vitro cell models and human research which suggested an important role for GABA(A)a6 along with b2, a1 and g2, in alcohol related phenotypes.3
In Canavan disease (inherited leukodystrophy), low level of expression of GABA(A)a6 was reported.4
Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat GABA(A)a6 Receptor. The Anti- GABA(A)a6 Receptor (extracellular) antibody (#AGA-004) can be used for Western Blot analysis and Immunohistochemical applications. It has been designed to recognize GABA(A)a6 Receptor from rat, mouse and human samples.