- 貨號 Cat. No.
- AGA-005
- 規格 Size
- 50 μl 0.2ml
Description
Anti-GABA(A) g2 (extracellular)
Epitope: |
Peptide QKSDDDYEDYASNKT(C), corresponding to residues 39-53 of rat GABA(A)g2 (Accession number P18508). |
Putative epitope location: |
Extracellular, N-terminus. |
Homology: |
Human, mouse, bovine-identical; Chick (13/15 residues identical). |
Reactivity Confirmed: |
Rat. |
Applications:
Western Blotting: |
Rat brain membrane (1:200). |
 |
Western blotting of rat brain membrane:
1. Anti-GABA(A)g2 (extracellular) antibody (#AGA-005) (1:200).
2. Anti-GABA(A)g2 (extracellular) antibody, preincubated with the control peptide antigen. |
|
Immunohistochemistry: |
Rat brain, formalin-fixed frozen section. |
 |
Staining with Anti-GABA (A) g2 (extracellular) antibody (#AGA-005) in rat globus pallidus and cresyl violet counter stain (pink-purple).
Neuronal nuclei were visualized (A, arrows). This pattern was completely blocked by pre-incubation with the antigen (B).
Calibration bar 50 um. |
1. Mihic, S.J. et al. (1994) J.Neurotransmit. Rev. 21, 127.
|
2. Whiting, P.J. et al. (1999) Neurochem. Int. 34, 379.
|
3. Ashcroft, F.M. (2000) Ion Channels and Disease Ed 1.
|
4. Wallace, R.H. et al. (2001) Nat. Genet. 28, 49.
|
5. Baulac, S. et al. (2001) Nat. Genet. 28, 46. |
The neurotransmitter GABA (g-aminobutyric acid) inhibits the activity of signal-receiving
neurons by interacting with the GABAA receptor on these cells.1 There are two major types of
GABA receptors: the ionotropic GABAA (GABAA R) and the metabotropic GABAB receptors.
GABAA R belongs to the ligand gated ion channel superfamily.1,2
It is a heteropentamer, with all of its five subunits contributing to the pore formation.
To date, eight subunit isoforms were cloned: a, b, g, d, e, P, q, and r.1
The native GABAA receptor, in most cases, consists of 2a, 2b and 1g subunit.
Three g subunits genes have been identified in mammals.
The binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel,
producing an increase in membrane conductance, resulting in inhibition of neural activity.2,3
Recently, a genetic linkage between familial epilepsy syndrome and mutations in the g2
subunit of the GABAA receptor have been demonstrated.4,5
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