Anti-GABA(A) a1 Receptor (extracellular)
- 貨號 Cat. No.
- AGA-001
- 規格 Size
- 50 μl 0.2ml
Description
Anti-GABA(A) a1 Receptor (extracellular)
| Product#: | AGA-001 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
Peptide QPSQD ELKDN TTVFT R(C), corresponding to amino acid residues 28-43 of rat GABA(A) a1 Receptor (Accession P18504). |
 |
| Putative epitope location: |
| Extracellular, N-terminus. |
| Homology: |
Mouse - identical; human and bovine - 16/17 amino acid residues identical; chicken - 14/17 amino acid residues identical. |
| Reactivity Confirmed: |
Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat brain lysate: 1.Anti-GABA(A) a1 receptor (extracellular) antibody (#AGA-001), (1:1000). 2.Anti-GABA(A) a1 receptor (extracellular) antibody preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of GABA(A)a1 receptor in mouse hippocampus |
 |
Immunohistochemical staining of GABA(A)a1 in mouse hippocampus using Anti-GABA(A)a1 receptor (extracellular) antibody (#AGA-001). A, Distribution of GABA receptor alpha1 (red), stained with Alomone antibody (#) in mouse hippocampus. B, distrubiton of glial fibrillary acidic protein (green). C, Merge of the two images indicates that distribution of GABA(A)a1 receptors is restricted to neurons and their processes. DAPI is used as the counterstain (blue). |
| References: |
| 1. Owens, D.F. and Kriegstein, A.R. (2002) Nat. Rev. Neurosci. 3, 715. |
| 2. Whiting, P.J. (1999) Neurochem. Int. 34, 387. |
| 3. Mihic, S.J. and Harris, R.A. (1997) Alcohol Health Res. World 21, 127. |
| 4. Neelands, T.R. et al. (1999) J. Neurosci. 19, 7057. |
| 5. Fuchs, K. and Celepirovic, N. (2002) J. Neurochem. 82, 1512. |
GABA (g-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system.1
The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily.2 GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells.3 Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity.2
GABAARs are heteropentamers, in which all five subunits contribute to pore formation. To date, eight subunit isoforms have been cloned: a, b, g, d, e,P, q, and r.1 Six a subunit isoforms have been found to exist in mammals (a1-a6). In most cases, native GABAA receptors consists of 2a, 2b, and 1g subunits. The a subunit is the most common and is expressed ubiquitously. It determines the affinities of GABAARs for allosteric ligands.
Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes.4 The a1 subunit is highly expressed in adulthood while the a2 subunit is highly expressed very early in rat brain development. Failure to complete the normal transition between the a-subunits that are highly expressed in early development (a2, a3, and a5) and those expressed in adulthood (a1) is suggested to play a major role in the development of temporal lobe epilepsy.5
Alomone Labs offers a highly specific antibody directed against an epitope at the extracellular N-terminus of the GABA(A) a1 receptor. The Anti-GABA(A) a1 Receptor (extracellular) antibody (#AGA-001)can be used in Western blot analysis and recognizes GABA(A) a1 receptor from rat and mouse samples.