- 貨號 Cat. No.
- AGB-002
- 規格 Size
- 50 μl 0.2ml
Description
Anti-GABA (B)R2
Epitope: |
Peptide CKDPIEDINSPEHIQRRLSL corresponding to residues 875-894 of human GABA (B)R2 (Accession number O75899). |
Putative epitope location: |
Intracellular, C-terminus.
|
Homology: |
Rat, mouse, bovine ?identical.
|
Reactivity Confirmed: |
Rat, mouse. |
Applications:
 |
Western blotting of rat brain membrane:
1. Anti-GABA (B)R2 antibody (#AGB-002) (1:200).
2. Anti-GABA (B)R2 antibody preincubated with the control peptide antigen. |
Immunohistochemistry: |
Expression of GABA (B)2 in mouse hippocampus |
 |
Immunohistochemical staining of frozen sections of mouse hippocampus. (A) Staining with Anti-GABA (B)R2 antibody (#AGB-002 ) (1:100) detects GABA (B)R2 (green) in layers including the stratum lacunosum moleculare (SLM) and stratum radiatum of the CA3 layer (white star). (B) Staining with mouse anti-GAP43 antibody identifies SLM. (C) Confocal merge of Anti-GABA (B)R2 and anti-GAP43 antibody staining suggests the presence of GABA (B)R2 in layers, but not in pyramidal neurons. |
1. Bowery, N.G. and Smart, T.G. (2006) Br. J. Pharmacol. 147, S109. |
2. Bettler, B. et al. (2004) Physiol. Rev. 84, 835. |
3. Boller, M. and Schmidt, M. (2003) J. Neurophysiol. 89, 2035. |
4. Robbins, M.J. et al. (2001) J. Neurosci. 21, 8043. |
5. Margeta-Mitrovic M. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 14649 |
6. Margeta-Mitrovic M. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 14643. |
7. http://www.expasy.org/uniprot/O75899. |
GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system and plays a crucial role in the modulation of neuronal activity.1,2
The GABA transmitter interacts with three types of receptors: the ionotropic receptors, GABA (A)R and GABA (C)R, and the metabotropic GABA (B) receptor, [GABA (B)R].3 The latter belongs to the G-protein-coupled receptor (GPCR) superfamily and mediates slow synaptic inhibition in the brain and spinal cord1,4.
The functional GABA (B) receptor [GABA (B)R] is a heterodimer consisting of two subunits, the GABA (B)R1 and the GABA (B)R2. These subunits were demonstrated to have complementary roles essential for the functional receptor.5,6
GABA (B)R2 has been shown to be essential for trafficking and for G-protein binding while the GABA (B)R1 subunit has been demonstrated to be important for agonist and antagonist binding. GABA (B)R2 is highly expressed in brain, especially in the cerebral cortex, thalamus, and hippocampus, spinal cord, and, to a lesser extent, in testis.7
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