Anti-GABA (B)R1 (extracellular)
- 貨號 Cat. No.
- AGB-001
- 規格 Size
- 50 μl 0.2ml
Description
Anti-GABA (B)R1 (extracellular)
| Product#: | AGB-001 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
Peptide CRGEREVVGPKVRK corresponding to residues 66-79 of rat GABA(B)R1(Accession Q9Z0U4). |
| Putative epitope location: |
Extracellular, N-terminus. |
 |
| Homology: |
Mouse, human, canine, bovine- identical. Specificity: Does not recognize alternative splice 1b. |
| Reactivity Confirmed: |
| Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blotting of rat brain membranes: 1. Anti-GABA(B)R1 (extracellular) antibody (#AGB-001) (1:200). 2. Anti-GABA(B)R1 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
| Expression of GABA(B)R1 mouse hippocampus |
 |
| Immunohistochemical staining of GABA (B)R1 in frozen sections of mouse hippocampus. (A) Staining with Anti-GABA (B)R1 (extracellular) antibody (#AGB-001) (1:100) detects GABA (B)R1 (green) in neurons in the CA3 field and in the dentate granule layer (short arrows), as well as in dendrites of CA3 pyramidal neurons (long arrows). (B) Staining with mouse anti-GAP43 antibody identifies stratum lacunosum moleculare (SLM). (C) Confocal merge of Anti-GABA (B)R1 and anti-GAP43 antibody staining suggests the presence of GABA (B)R1 in pyramidal neurons. |
| References: |
| 1. Bowery, N.G. and Smart, T.G. (2006) Br. J. Pharmacol. 147, S109. |
| 2. Bettler, B. et al. (2004) Physiol. Rev. 84, 835. |
| 3. Boller, M. and Schmidt, M. (2003) J. Neurophysiol. 89, 2035. |
| 4. Robbins, M.J. et al. (2001) J. Neurosci. 21, 8043. |
| 5. Margeta-Mitrovic M. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 14649. |
| 6. Margeta-Mitrovic M. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 14643. |
| 7. http://www.iuphar-db.org/GPCR/ChapterMenuForward?chapterID=1276 |
| 8. http://www.expasy.org/uniprot/Q9Z0U4 |
GABA (gamma-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system and plays a crucial role in the modulation of neuronal activity.1,2
The GABA transmitter interacts with three types of receptors: the ionotropic receptors, GABA (A)R and GABA (C)R, and the metabotropic GABA (B) receptor, [GABA (B)R].3 The latter belongs to the G-protein-coupled receptor superfamily and mediates slow synaptic inhibition in the brain and spinal cord1,4.
The functional GABA (B) receptor [GABA (B)R] is a heterodimer consisting of two subunits, the GABA (B)R1 and the GABA (B)R2. These subunits were demonstrated to have complementary roles essential for the functional receptor.5,6 The GABA (B)R1 subunit was demonstrated to be important for agonist and antagonist binding, while GABA (B)R2 was shown to be essential for trafficking and for G-protein binding.
To date, eight alternatively spliced isoforms of GABA (B)R1 have been proposed. These are named 1a-1h, of which 1a and 1b are the most prominent. Only 1a, 1b, and 1c appear to act as functional subunits.6,7 They are widespread, being expressed in testis, stomach, spinal cord, and brain. Isoform 1b is also expressed in kidney and liver.8