Anti-GABA (A) a3 Receptor (extracellular)
- 貨號 Cat. No.
- AGA-003
- 規格 Size
- 50 μl 0.2ml
Description
Anti-GABA (A) a3 Receptor (extracellular)
| Product#: | AGA-003 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
Peptide QGESR RQEPG DFVKQ (C), corresponding to amino acid residues 29-43 of human GABA(A)a3 receptor (Accession P34903). |
 |
| Putative epitope location: |
| Extracellular, N-terminus. |
| Homology: |
Rat, mouse - identical; bovine - 14/15 amino acid residues identical. |
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
 | Western blot analysis of GABA(A)a3 (extracellular) in rat brain membrane: 1. Anti-GABA(A) a3 receptor (extracellular) antibody (#AGA-003), (1:200). 2. Anti-GABA(A) a3 receptor (extracellular) antibody preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of GABA(A)a3 receptor in rat cerebellum |
 |
Immunohistochemical staining of GABA(A)a3 in rat cerebellum using Anti-GABA(A)a3 receptor (extracellular) (#AGA-003) (green) (1:100). GABA(A)a3 receptor was localized to the molecular layer (white asterik) and a portion of the purkinje cell body (arrows), which was outlined by axonal staining using mouse anti neurofilament 200 (red). |
| References: |
| 1. Mihic, S.J. and Harris, R.A. (1997) Alcohol Health Res. World 21, 127. |
| 2. Whiting, P.J. (1999) Neurochem. Int. 34, 387. |
| 3. Fuchs, K. and Celepirovic, N. (2002) J. Neurochem. 82, 1512. |
The neurotransmitter GABA (g-aminobutiric acid) inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells.1 Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity.2
There are two major types of GABA receptors: the ionotropic GABAA receptors (GABAAR) and the metabotropic GABAB receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily.1,2
GABAARs are heteropentamers, in which all five subunits contribute to formation of the pore.
Eight subunit isoforms have been cloned: a, b, g, d, e, p, q, and r.1
Six a subunits isoforms (a1-a6) have been shown to exist in mammals.
In most cases, the native GABAA receptors consist of 2a, 2b, and 1g subunits.
The a3-subunit is highly expressed during development (along with a2 and a5) and then declines in adulthood, where the a1-subunit becomes predominant. The failure to complete this switch could be a major predispositional factor in the development of temporal lobe epilepsy.3
Alomone Labs offers a highly specific antibody directed against an epitope at the extracellular N-terminus of the GABA(A) a3 receptor. The Anti-GABA(A) a3 receptor(extracellular) antibody(#AGA-003)can be used in Western blot analysis and Immunohistochemical applications. It has been designed to recognizes GABA(A) a3 receptor from rat, mouse, and human samples.