Anti-G6PD Antibody

Anti-G6PD Antibody LS-B3587

G6PD Mouse anti-Human Monoclonal (AT2F6) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B3587 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3587 was determined to be 5 ug/ml. Western Blot: Cell lysates of MCF7 (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human G6PD (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Learn more

G6PD Antibody Details

Target: Human G6PD

Host: Mouse

Reactivity: Human

Clonality: IgG2b,k Monoclonal [AT2F6] 

Purification: Protein G Column

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (5)

Western blot (WB) (1:1000 - 1:2000)

ELISA

Optimal dilution to be determined by the researcher.

 

G6PD Antibody Specificity

Immunogen: G6PD antibody was raised against recombinant human G6PD (35-506aa) purified from E. coli

Specificity: human G6PD (35-506aa)

G6PD Antibody Packaging

Presentation: PBS, pH 7.4, 0.1% sodium azide

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For research use only.