Anti-FN1 / Fibronectin Antibody

Anti-FN1 / Fibronectin Antibody LS-B382

FN1 / Fibronectin Rabbit anti-Human Polyclonal Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B382 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B382 was determined to be 1.25 ug/ml. Learn more

FN1 / Fibronectin Antibody Details

 

 

Target:

Human FN1 / Fibronectin

 

Host:

Rabbit

 

Reactivity:

Human

 

Clonality:

 Polyclonal

 

Purification:

Immunoaffinity Chromatography

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (1.25)

 

Western blot (WB) (1:5000 - 1:10000)

 

Immunoprecipitation (IP) (1:100)

 

ELISA (1:5000 - 1:10000)

 

Optimal dilution to be determined by the researcher.

 

FN1 / Fibronectin Antibody Specificity

 

Specificity:

Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single-band purity was assessed by SDS-PAGE.

FN1 / Fibronectin Antibody Packaging

 

Presentation:

0.02 M K phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.

 

Storage:

Store vial at 4 C prior to opening. This product is stable at 4 C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20 C or below.

 

Restrictions:

For research use only.