Anti-EPCAM Antibody

Anti-EPCAM Antibody LS-B4511

EPCAM Mouse anti-Human Monoclonal (VU-1D9) Antibody

An IHC-plusTM Antibody

 Flow Cytometry. Recommended dilution: 1-3 ug/ml. Immunohistochemistry (paraffin sections). Recommended dilution: 5-10 ug/ml. Positive control: colon epithelium. Application note: antigen retrieval: heat (sodium citrate) + trypsin; mAb incubation: 1 hour / RT; detection DAB. Immunoprecipitation. Recommended dilution: 1-2 ug / 100-500 ug of protein in 1 ml lysate Western Blotting. Recommended dilution: 1-4 ug/ml Immunohistochemistry (frozen sections). Immunocytochemistry. Recommended dilution: 4 ug/ml. Learn more

EPCAM Antibody Details

 

 

Target:

Human EPCAM

 

Host:

Mouse

 

Reactivity:

Human

 

Clonality:

IgG1 Monoclonal [VU-1D9]

 

Purification:

Protein A Column

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10)

 

Immunohistochemistry - Frozen (IHC-Fr)

 

Immunocytochemistry (ICC) (4)

 

Western blot (WB) (1 - 4)

 

Immunoprecipitation (IP)

 

Flow Cytometry (Flo) (1 - 3)

 

Optimal dilution to be determined by the researcher.

 

EPCAM Antibody Specificity

 

Immunogen:

EPCAM antibody was raised against small cell lung carcinoma cell line H69.

 

Specificity:

The mouse monoclonal antibody VU-1D9 recognizes an epitope within EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.

EPCAM Antibody Packaging

 

Presentation:

PBS, 15 mM sodium azide, pH 7.4

 

Storage:

Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

 

Restrictions:

For research use only.